Abstract

Soybean is the world’s leading economic oilseed crop. Drought stress is a major constraint on the growth and yield stability of soybean. Here, wild soybean (Glycine soja Siebold & Zucc.) was found to be more drought tolerant than cultivated soybean (Glycine max (L.) Merr.) owing to morphological changes at the whole-plant level when subjected to 5% PEG-6000 treatment. Additionally, differential metabolites between two soybean genotypes seedlings leaves were analyzed at the cellular level using a gas chromatography-mass spectrometry-based metabolomics method. The root lengths of wild soybean increased and a high root/shoot ratio was maintained under drought stress conditions. In addition, the drought tolerance of wild soybean resulted from significantly greater levels of favorable metabolites, such as aromatic and serine family amino acids, as well as sugar and polylols involved in mannose and galactose metabolism, favorable secondary metabolites, and organic and fatty acids compared with cultivated soybean. At the same time, wild soybeans could maintain a stable TCA cycle and significantly enhance glycolysis to produce more energy and enhance the phosphate pentose pathway to create more reducing power. Our experimental results provide an important reference for the breeding of wild soybeans in arid environments, as well as methodological for utilizing wild soybeans and improving cultivated soybeans.

中文翻译：

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干旱胁迫下两种大豆基因型代谢组的变化

摘要

大豆是世界领先的经济油料作物。干旱胁迫是制约大豆生长和产量稳定的主要因素。在这里，发现野生大豆（Glycine soja Siebold＆Zucc。）比栽培大豆（Glycine max（L.）Merr。）是因为在进行5％PEG-6000处理时，整个植物的形态发生了变化。此外，使用基于气相色谱-质谱的代谢组学方法，在细胞水平上分析了两种基因型大豆幼苗叶片之间的差异代谢物。在干旱胁迫条件下，野生大豆的根长增加，并且保持高根/茎比。此外，野生大豆的耐旱性是由于有益代谢物（例如芳香族和丝氨酸家族氨基酸）水平高得多，以及参与甘露糖和半乳糖代谢的糖和多元醇，有利的次生代谢产物以及有机和脂肪酸的水平与栽培大豆。同时，野生大豆可以维持稳定的TCA周期，并显着增强糖酵解作用以产生更多能量，并增强磷酸戊糖途径产生更多的还原能力。我们的实验结果为干旱环境下野生大豆的育种以及利用野生大豆和改良栽培大豆的方法学提供了重要的参考。