Abstract

In this study, the polysome profiling method was used for the separation of mRNA depending on their loading by ribosomes into polysomal and monosomal fractions. Separation of pools of such mRNA and analysis of transcripts (mRNA), which are characterized by a constant level of transcription in a wide range of absolute values at all stages of plant ontogenesis and associated with each pool of mRNA due to RNA sequencing, allowed for obtaining an idea about the translational efficiency of individual mRNA. The consequent in silico analysis allowed performing a search for regulatory contexts in the 5'-region of mRNA of Arabidopsis thaliana plants that may be potentially important for efficient mRNA translation. The results of the study revealed that pyrimidine dinucleotides and motifs are characteristic of a 5'-untranslated mRNA region with high translation efficiency, whereas purine dinucleotides and motifs are associated with transcripts with low translational efficiency.

中文翻译：

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拟南芥植物mRNA 5'-区的调控环境及其在翻译效率中的作用

摘要

在这项研究中，多核糖体谱分析方法用于分离mRNA，具体取决于核糖体将其装载到多核糖体和单核糖体组分中。分离此类mRNA的库并进行转录本（mRNA）分析，其特征是在植物本体形成的各个阶段，在各种绝对值中均具有恒定的转录水平，并且由于RNA测序而与每个mRNA库相关联，了解有关单个mRNA的翻译效率的想法。随后的计算机模拟分析允许在拟南芥的mRNA 5'-区域中进行调控背景的搜索。对于有效的mRNA翻译可能潜在重要的植物。研究结果表明，嘧啶二核苷酸和基序是5'-非翻译mRNA区域的特征，翻译效率高，而嘌呤二核苷酸和基序与转录物相关，翻译效率低。