WaterControl is a solvent suppression method based on WATERGATE and PGSTE and is very efficient in selectively reducing the solvent signal in 1D pulse-acquire and 2D NOESY of protein solutions. In this study, the WaterControl technique was appended to two common 2D NMR methods used in resonance assignment of proteins, namely TOCSY and CLIP-COSY. Similar to that observed in regular 1D pulse-acquire and 2D NOESY, the incorporation of WaterControl in these 2D methods led to excellent solvent suppression superior to that obtained using W3- or W5-based WATERGATE sequences. The water signal was essentially eliminated in the TOCSY and CLIP-COSY with WaterControl while useful cross peaks around the water resonance at ω2 were preserved. This is in contrast to the 2D spectra obtained from the corresponding WATERGATE containing sequences, where these cross peaks in the ω2 region are usually suppressed together with the water resonance. These new WaterControl sequences provide significantly improved water suppression thereby facilitating protein NMR studies.

中文翻译：

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WaterControl在TOCSY和COYY实验中的应用。

WaterControl是一种基于WATERGATE和PGSTE的溶剂抑制方法，在选择性减少蛋白质溶液的1D脉冲采集和2D NOESY过程中的溶剂信号方面非常有效。在这项研究中，WaterControl技术被附加到蛋白质共振分配所使用的两种常见的2D NMR方法中，即TOCSY和CLIP-COSY。与在常规1D脉冲采集和2D NOESY中观察到的相似，在这些2D方法中并入WaterControl导致优异的溶剂抑制效果优于使用基于W3或W5的WATERGATE序列获得的抑制效果。使用WaterControl在TOCSY和CLIP-COSY中基本消除了水信号，同时保留了在ω2处水共振附近有用的交叉峰。这与从相应的WATERGATE包含序列获得的2D光谱相反，在ω2区域的这些交叉峰通常与水共振一起被抑制。这些新的WaterControl序列可显着改善水抑制性能，从而促进蛋白质NMR研究。