PURPOSE This study aimed to explore the aneuploidy of blastocysts derived from single pronuclear (1PN) zygotes, almost 75% of which were regarded as diploid, using array CGH and examine the pregnancy outcomes. METHODS Embryonic aneuploidy screening of sixteen embryos from 1PN zygotes and sixteen embryos from 2PN zygotes was performed using array CGH in study 1. In addition, the reproductive outcome of 1761 single blastocysts, after untested frozen-thawed blastocyst transfer in IVF/ICSI patients, was retrospectively analyzed and compared between the 1PN and 2PN groups in study 2. RESULTS The aneuploidy rates were 30.8% (4/13) in 1PN IVF, 33.3% (1/3) in 1PN ICSI, 46.2% (6/13) in 2PN IVF, and 100% (3/3) in 2PN ICSI. The 1PN group achieved clinical pregnancy in 25.0% (7/28) of IVF and 30.0% (3/10) of ICSI, whereas these rates in the 2PN control group were 44.6% (557/1250) of IVF and 37.4% (177/473) of ICSI. No miscarriage occurred in the pregnancies from 1PN zygotes, whereas the rates of miscarriage in the 2PN control group were 22.6% (126/557) in IVF and 22.2% (39/176) in ICSI. The delivery rate was similar in all groups. Ten deliveries in the 1PN group showed no newborn malformation. CONCLUSION Within the limits of the small sample size, our results suggest that the aneuploidy and delivery rates of the blastocysts derived from 1PN zygotes are the same as those derived from 2PN zygotes. Blastocysts derived from 1PN zygotes may be used clinically and could increase the chance of pregnancy.

中文翻译：

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通过阵列 CGH 对单个原核受精卵衍生的囊胚进行染色体分析，并通过单个原核受精卵移植进行临床结果。

目的本研究旨在利用阵列 CGH 探讨单原核 (1PN) 受精卵来源的囊胚的非整倍性，其中几乎 75% 被视为二倍体，并检查妊娠结局。方法 在研究 1 中，使用阵列 CGH 对来自 1PN 受精卵的 16 个胚胎和来自 2PN 受精卵的 16 个胚胎进行胚胎非整倍性筛查。此外，在 IVF/ICSI 患者中进行未经测试的冻融囊胚移植后，1761 个单个囊胚的生殖结果为回顾性分析比较研究 2 中 1PN 和 2PN 组。 结果 1PN IVF 中非整倍体率为 30.8%（4/13），1PN ICSI 中为 33.3%（1/3），2PN 中为 46.2%（6/13） IVF 和 100% (3/3) 2PN ICSI。1PN 组 IVF 的临床妊娠率为 25.0% (7/28)，ICSI 的临床妊娠率为 30.0% (3/10)，而 2PN 对照组 IVF 的临床妊娠率为 44.6% (557/1250)，ICSI 的临床妊娠率为 37.4% (177)。 /473）ICSI。1PN受精卵妊娠未发生流产，而2PN对照组IVF流产率为22.6%（126/557），ICSI流产率为22.2%（39/176）。所有组的分娩率相似。1PN 组的 10 次分娩未见新生儿畸形。结论 在小样本量的范围内，我们的结果表明，1PN 受精卵衍生的囊胚的非整倍性和分娩率与 2PN 受精卵衍生的囊胚相同。1PN 受精卵衍生的囊胚可用于临床，并可增加怀孕的机会。