Thirty isolates of Pseudomonas syringae other than pathovar (pv.) phaseolicola, recovered from beans (18) and weeds (12) in a Northern Spanish region, were studied by a polyphasic approach, including phenotypic tests, detection of levansucrase and toxin (syringomycin and syringopeptin) genes, typing by RAPD (Random Amplified Polymorphic DNA) and PFGE (Pulsed-Field Gel Electrophoresis). The isolates were phenotypically heterogeneous, and both RAPD and PFGE, probed to be highly discriminative, as they were almost able to differentiate at the level of isolate. Phylogenetic analysis based on conserved genes of the core genome (rpoD, gltA and gyrB) distributed the isolates in three different clades (2A, 2B and 2C) belonging to phylogroup 2 of the P. syringae complex. According to toxin production, 18 of them, within clade 2B, were assigned to P. syringae pv. syringae and five of these caused symptoms after experimental inoculation on bean pods. Four of the five pathogenic isolates were obtained from weeds, supporting the role of weeds associated with bean crops, particularly Cyperus rotundus, Malva sylvestris, and Polygonum lapathifolium, as a reservoir of the pathogen. In conclusion, results of this study suggest that apart from P. syringae pv. phaseolicola, P. syringae isolates circulating in our region are mostly epiphytes, and support the role of weeds associated with bean crops as reservoir of pathogenic strains of P. syringae pv. syringae.

通过多相方法研究了从西班牙北部地区的豆类（18）和杂草（12）中回收的除菜豆（pv。）菜豆以外的丁香假单胞菌的30种分离物，包括表型测试，检测葡糖蔗糖酶和毒素（丁香霉素和syringopeptin）基因，通过RAPD（随机扩增多态性DNA）和PFGE（脉冲场凝胶电泳）进行分型。这些分离株在表型上是异质的，并且探测到RAPD和PFGE都具有高度区分性，因为它们几乎能够在分离株的水平上进行区分。基于核心基因组保守基因（rpoD，gltA和gyrB的系统发育分析））将分离物分布在丁香假单胞菌复合体的第2系统族的三个不同进化枝（2A，2B和2C）中。根据毒素的产生，在进化枝2B内，其中有18种被分配给丁香假单胞菌PV。丁香科和其中五种在豆荚上进行实验性接种后引起症状。五种病原体中有四种是从杂草中获得的，支持了与豆类作物相关的杂草，尤其是香附子，锦葵和何首乌作为病原体的贮藏库。总之，这项研究的结果表明，除了丁香假单胞菌。菜豆，丁香假单胞菌在我们地区流通的分离株大多是附生植物，它们支持与豆类作物相关的杂草作为丁香假单胞菌pv致病菌株的贮藏库的作用。丁香科。