Spirulina platensis, when grown under stress, may alter its metabolic pathways, leading to carbohydrate accumulation. Research on the combined effects of stress factors such as UV radiation, photoperiod, and light, and the influence of micronutrients (Ca, Fe, and Mg) on microalgae carbohydrate composition is scarce. The aim of the present study was to evaluate the effects of combined cell stress factors on the microalgal growth and biochemical composition of S. platensis, to apply the biomass in the context of biorefineries. Assays were performed at two cultivation stages: in the first stage, Zarrouk medium (50%) was used until the end of the exponential growth phase (18 days) and, in the second stage, the cells obtained in the first stage were centrifuged and recultivated in 20% Zarrouk’s medium under physical (UV, photoperiod/light, NaCl) and nutritional stress (limitation of Ca, Fe, and Mg concentrations in 20% Zarrouk). The highest carbohydrate yields (27.84 mg L⁻¹ day⁻¹) were obtained in the photoperiod/light intensity-stressed cultures in the medium with nutrient limitation. The study outcomes showed the methods to increase carbohydrate synthesis in cultures under optimized conditions. Our results showed that nutrient limitation by 20% Zarrouk combined with higher light intensity (67.5 μmol photons m⁻² s⁻¹) and photoperiod (18-h/6-h light/dark cycles) is an efficient strategy to achieve higher intracellular concentrations (59.71%) and carbohydrate yields (55.85 mg L⁻¹ day⁻¹) in a discontinuous culture method.

螺旋藻在压力下生长时，可能会改变其代谢途径，导致碳水化合物积累。缺乏对诸如紫外线，光周期和光等压力因素的综合影响以及微量营养元素（Ca，Fe和Mg）对微藻碳水化合物组成的影响的研究。本研究的目的是评估细胞应激综合因素对S. platensis微藻生长和生化组成的影响。，将生物质应用于生物炼油厂。在两个培养阶段进行测定：在第一阶段，使用Zarrouk培养基（50％）直至指数生长期结束（18天）；在第二阶段，将在第一阶段获得的细胞离心并在物理（紫外线，光周期/光，NaCl）和营养胁迫（限制20％Zarrouk中的Ca，Fe和Mg浓度）下，在20％Zarrouk培养基中重新培养。最高的碳水化合物产量（27.84 mg L ^-1 天^-1）是在受营养限制的培养基中于光周期/光照强度培养中获得的。研究结果显示了在优化条件下增加培养物中碳水化合物合成的方法。我们的结果表明，通过20％Zarrouk限制营养，并结合更高的光强度（67.5μmol光子m ^-2  s ^-1）和光周期（18-h / 6-h光照/黑暗周期）是实现更高细胞内浓度的有效策略不连续培养方法的收率（59.71％）和碳水化合物收率（55.85 mg L ^-1  day ^-1）。