In this study, we evaluated polyurethane foam (PF), volcanic rock (VR), and a modified plastic cap (MPC) as supports for the immobilization of organophosphorus (OP) pesticide-degrading actinobacterial strains. The colonization and activity of four streptomycetes were favoured by PF, which was selected as the carrier to use in a continuous stirred tank bioreactor (CSTR) that can be operated at increasing inflows of a pesticide mixture that contains the insecticides chlorpyrifos (CP) and diazinon (DZ). Our results demonstrate that the CSTR can be operated at flow rates of 10 and 40 mL h-1 with greater than 85% removal of the pesticides in the short term. A significant decrease in the efficiency of CP removal was observed at the highest inflows into the reactor. The CP and DZ loading rates in the bioreactor ranged from 0.44 to 1.68 mg L-1 h-1 and from 0.50 to 2.17 mg L-1 h-1, respectively. Although the treated wastewater exhibited moderate toxicity for Raphanus sativus, a bioreactor inoculated with a mixed culture formed by Streptomyces spp. strains AC5, AC9, GA11 and ISP13 may provide an effective biotechnological strategy for the reduction of OP pesticide residues produced during agronomic and manufacturing practices and therefore prevent environmental pesticidal pollution.

中文翻译：

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使用固定化放线菌混合培养物去除有机磷农药的连续搅拌釜式生物反应器的性能。

在这项研究中，我们评估了聚氨酯泡沫（PF），火山岩（VR）和改性塑料盖（MPC）作为固定化有机磷（OP）降解农药的放线菌菌株的支持。PF促进了四种链霉菌的定殖和活性，PF被选作连续搅拌罐生物反应器（CSTR）的载体，该反应器可在含有杀虫剂毒死rif（CP）和二嗪农的农药混合物增加流入量的情况下运行（DZ）。我们的结果表明，CSTR可以在10和40 mL h-1的流速下运行，并且在短期内可去除超过85％的农药。在流入反应器的流量最高时，观察到了CP去除效率的显着下降。生物反应器中CP和DZ的加载速率为0.44至1。分别为68 mg L-1 h-1和0.50至2.17 mg L-1 h-1。尽管处理后的废水对萝卜丝具有中等毒性，但是生物反应器接种了链霉菌形成的混合培养物。AC5，AC9，GA11和ISP13菌株可提供有效的生物技术策略，以减少农艺和生产过程中产生的OP农药残留，从而防止环境农药污染。