Due to its unique mechanical properties, spider silk shows great promise as a strong super-thin fiber in many fields. Although progress has been made in the field of synthesizing spider-silk fiber from recombinant spidroin (spider silk protein) in the last few decades, methods to obtain synthetic spider-silk fibers as tough as natural silk from small-sized recombinant protein with a simple spinning process have eluded scientists. In this paper, a recombinant spidroin (MW: 93.4 kDa) was used to spin tough synthetic spider-silk fibers with a simple wet-spinning process. Titanium oxide incorporation and formaldehyde cross-linking were used to improve the mechanical properties of synthetic spider-silk fibers. Fibers treated with incorporation or/and cross-linking varied in microstructure, strength and extensibility while all exhibited enhanced strength and toughness. In particular, one fiber possessed a toughness of 249 ± 22 MJ/m³. This paper presents a new method to successfully spin tough spider-silk fibers in a simple way.

由于其独特的机械性能，蜘蛛丝在许多领域都显示出作为强力超薄纤维的广阔前景。尽管在过去的几十年中，在利用重组蜘蛛丝蛋白（蜘蛛丝蛋白）合成蜘蛛丝纤维方面取得了进展，但是通过简单的方法从小型重组蛋白中获得与天然丝一样坚韧的合成蜘蛛丝纤维的方法已经有了。纺丝过程还没有引起科学家的注意。在本文中，使用一种简单的湿纺工艺，将重组蜘蛛丝蛋白（MW：93.4 kDa）纺成坚韧的合成蜘蛛丝纤维。掺入二氧化钛和甲醛交联可改善合成蜘蛛丝纤维的机械性能。经过掺入或/和交联处理的纤维的微观结构各不相同，强度和可延展性，同时都表现出增强的强度和韧性。特别是，一种纤维的韧性为249±22 MJ / m³。本文提出了一种通过简单方法成功纺出坚韧的蜘蛛丝纤维的新方法。