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Revisiting seed transmission of ‘Candidatus Liberibacter asiaticus’ in different citrus species and hybrids
Tropical Plant Pathology ( IF 1.5 ) Pub Date : 2020-05-14 , DOI: 10.1007/s40858-020-00348-7
Thiago Z. Bagio , Marcelo G. Canteri , Rui P. Leite Júnior

Huanglongbing (HLB) caused by ‘ Candidatus Liberibacter spp.’ is the most destructive disease for citrus production. These bacteria are disseminated naturally by insect vectors, such as the psyllid Diaphorina citri . However, the transmission of the bacteria vertically from seeds to seedlings has been controversial. Therefore, the objective of this study was to re-investigate the vertical transmission of the bacterium ‘ Ca . Liberibacter asiaticus’ (CLas) from seeds to seedlings. Seeds of ‘Rangpur’ lime, ‘Swingle’ citrumelo, ‘Ponkan’ mandarin and the sweet oranges ‘Folha Murcha’, ‘IAPAR-73’, ‘Pera Rio’, ‘Shamouti’ and ‘Valencia’ were included in the study. Besides the seeds, 3493 citrus seedlings were also examined for the presence of the bacterium and for the development of HLB symptoms. The presence of the bacterium in different parts of the seeds and in citrus seedlings was examined by standard PCR. CLas was detected in all parts of the seeds, including seed coat or testa, tegmen, and endosperm plus embryos of all citrus cultivars, except for ‘Shamouti’ sweet orange. Although transient, the presence of the HLB bacterium was also detected in seedlings of ‘Ponkan’ mandarin and ‘Folha Murcha’ sweet orange obtained from seeds of HLB-infected trees up to 18 months after seedlings transplanting. However, no typical symptoms of HLB were observed in the citrus seedlings. Although CLas may infect the citrus seeds, the results of this study do not support the vertical transmission of viable cells of CLas from seeds to seedlings.

中文翻译:

重新审视'Candidatus Liberibacter asiaticus'在不同柑橘物种和杂交种中的种子传播

由“自由念珠菌属”引起的黄龙病 (HLB)。是柑橘生产中最具破坏性的病害。这些细菌通过昆虫载体自然传播,例如木虱 Diaphorina citri。然而,细菌从种子垂直传播到幼苗一直存在争议。因此,本研究的目的是重新研究细菌 'Ca 的垂直传播。Liberibacter asiaticus' (CLas) 从种子到幼苗。'Rangpur' 酸橙、'Swingle' citrumelo、'Ponkan' 柑橘和甜橙 'Folha Murcha'、'IAPAR-73'、'Pera Rio'、'Shamouti' 和 'Valencia' 的种子都包括在研究中。除种子外,还检查了 3493 株柑橘幼苗是否存在细菌和 HLB 症状。通过标准 PCR 检测种子不同部位和柑橘幼苗中细菌的存在。在种子的所有部分都检测到 CLas,包括所有柑橘栽培品种的种皮或种皮、tegmen 和胚乳加胚,除了 'Shamouti' 甜橙。尽管是短暂的,但在从受 HLB 感染的树木的种子中获得的 'Ponkan' 柑橘和 'Folha Murcha' 甜橙的幼苗中也检测到了 HLB 细菌的存在,该幼苗移植后长达 18 个月。然而,在柑橘幼苗中没有观察到典型的 HLB 症状。尽管 CLas 可能会感染柑橘种子,但本研究的结果并不支持 CLas 的活细胞从种子到幼苗的垂直传播。包括种皮或种皮、tegmen 和胚乳以及所有柑橘栽培品种的胚,“Shamouti”甜橙除外。尽管是短暂的,但在从受 HLB 感染的树木的种子中获得的 'Ponkan' 柑橘和 'Folha Murcha' 甜橙的幼苗中也检测到了 HLB 细菌的存在,该幼苗移植后长达 18 个月。然而,在柑橘幼苗中没有观察到典型的 HLB 症状。尽管 CLas 可能会感染柑橘种子,但本研究的结果并不支持 CLas 的活细胞从种子到幼苗的垂直传播。包括种皮或种皮、tegmen 和胚乳以及所有柑橘栽培品种的胚,“Shamouti”甜橙除外。尽管是短暂的,但在从受 HLB 感染的树木的种子中获得的 'Ponkan' 柑橘和 'Folha Murcha' 甜橙的幼苗中也检测到了 HLB 细菌的存在,该幼苗移植后长达 18 个月。然而,在柑橘幼苗中没有观察到典型的 HLB 症状。尽管 CLas 可能会感染柑橘种子,但本研究的结果并不支持 CLas 的活细胞从种子到幼苗的垂直传播。在从受 HLB 感染的树木的种子中获得的 'Ponkan' 柑橘和 'Folha Murcha' 甜橙的幼苗中也检测到 HLB 细菌的存在,这些幼苗是在幼苗移栽后长达 18 个月的时间。然而,在柑橘幼苗中没有观察到典型的 HLB 症状。尽管 CLas 可能会感染柑橘种子,但本研究的结果并不支持 CLas 的活细胞从种子到幼苗的垂直传播。在从受 HLB 感染的树木的种子中获得的 'Ponkan' 柑橘和 'Folha Murcha' 甜橙的幼苗中也检测到 HLB 细菌的存在,这些幼苗是在幼苗移栽后长达 18 个月的时间。然而,在柑橘幼苗中没有观察到典型的 HLB 症状。尽管 CLas 可能会感染柑橘种子,但本研究的结果并不支持 CLas 的活细胞从种子到幼苗的垂直传播。
更新日期:2020-05-14
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