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Identification of copper-regulated proteins in an oceanic diatom, Thalassiosira oceanica 1005.
Metallomics ( IF 2.9 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0mt00033g Liangliang Kong 1 , Neil M Price 2
Metallomics ( IF 2.9 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0mt00033g Liangliang Kong 1 , Neil M Price 2
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Copper (Cu) is an essential cofactor of photosynthetic and respiratory redox proteins in phytoplankton and a scarce resource in parts of the open sea. Although its importance for growth is well recognized, the molecular mechanisms by which phytoplankton respond and acclimate to Cu deficiency are not well known. In this study, we identified the dominant Cu-regulated proteins and measured key physiological traits of Thalassiosira oceanica (CCMP 1005) under Cu-limiting and sufficient conditions. Growth limitation of T. oceanica occurred at environmentally relevant Cu concentrations (1 nM) as a result of decreased photosynthetic efficiency (ΦPSII). In Cu-limited cells, levels of plastocyanin decreased by 3-fold compared to Cu-replete cells and rates of maximum photosynthetic electron transport were reduced. Proteins associated with light harvesting complexes also declined in response to Cu limitation, presumably to adjust to reduced photosynthetic electron flow and to avoid photodamage to the photosystems. Key enzymes involved in carbon and nitrogen assimilation were down-regulated in low-Cu cells, as were steady state rates of C and N uptake. Relatively fewer proteins were up-regulated by Cu limitation, but among them were two enzymes involved in fatty acid oxidation (FAO). The increase in FAO may be a sign of increased turnover of cellular lipids caused by damage from oxidative stress. A putative transcription factor containing three, repetitive methionine motifs (MpgMgggM; MpgMggM) increased significantly in Cu-limited cells. The collective results provide a general description of how plastocyanin-dependent diatoms adjust metabolism to cope with chronic Cu deficiency.
中文翻译：
海洋硅藻中铜调节蛋白的鉴定，Thalassiosira Oceanica 1005。
铜 (Cu) 是浮游植物中光合和呼吸氧化还原蛋白的重要辅助因子，也是部分公海的稀缺资源。尽管它对生长的重要性已得到广泛认可，但浮游植物对铜缺乏反应和适应的分子机制尚不清楚。在这项研究中，我们确定了主要的 Cu 调节蛋白，并在 Cu 限制和充分条件下测量了Thalassiosira Oceanica (CCMP 1005) 的关键生理特征。的生长限制T.大洋洲发生在环境相关的Cu浓度（1nM）的作为结果的降低的光合效率（Φ PSII）。在铜限制的细胞中，与富铜细胞相比，质体蓝素的水平降低了 3 倍，并且最大光合电子传输速率降低。与光捕获复合物相关的蛋白质也因铜限制而下降，可能是为了适应减少的光合电子流并避免对光系统的光损伤。参与碳和氮同化的关键酶在低铜细胞中被下调，C 和 N 吸收的稳态速率也是如此。Cu限制上调的蛋白质相对较少，但其中有两种参与脂肪酸氧化（FAO）的酶。FAO 的增加可能是由氧化应激损伤引起的细胞脂质周转增加的迹象。包含三个重复甲硫氨酸基序的推定转录因子 (MpgMgggM; MpgMggM) 在铜限制细胞中显着增加。集体结果提供了质体蓝素依赖性硅藻如何调节代谢以应对慢性铜缺乏的一般描述。
更新日期：2020-07-22
中文翻译：
海洋硅藻中铜调节蛋白的鉴定，Thalassiosira Oceanica 1005。
铜 (Cu) 是浮游植物中光合和呼吸氧化还原蛋白的重要辅助因子，也是部分公海的稀缺资源。尽管它对生长的重要性已得到广泛认可，但浮游植物对铜缺乏反应和适应的分子机制尚不清楚。在这项研究中，我们确定了主要的 Cu 调节蛋白，并在 Cu 限制和充分条件下测量了Thalassiosira Oceanica (CCMP 1005) 的关键生理特征。的生长限制T.大洋洲发生在环境相关的Cu浓度（1nM）的作为结果的降低的光合效率（Φ PSII）。在铜限制的细胞中，与富铜细胞相比，质体蓝素的水平降低了 3 倍，并且最大光合电子传输速率降低。与光捕获复合物相关的蛋白质也因铜限制而下降，可能是为了适应减少的光合电子流并避免对光系统的光损伤。参与碳和氮同化的关键酶在低铜细胞中被下调，C 和 N 吸收的稳态速率也是如此。Cu限制上调的蛋白质相对较少，但其中有两种参与脂肪酸氧化（FAO）的酶。FAO 的增加可能是由氧化应激损伤引起的细胞脂质周转增加的迹象。包含三个重复甲硫氨酸基序的推定转录因子 (MpgMgggM; MpgMggM) 在铜限制细胞中显着增加。集体结果提供了质体蓝素依赖性硅藻如何调节代谢以应对慢性铜缺乏的一般描述。
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