Oleaginous yeasts can accumulate intracellular lipid bodies or triacylglycerides (TAGs) under nutrient limiting conditions. TAGs derived from those yeast strains are considered as an alternative to conventional plant-based oils for biodiesel production. In this study, we attempt to isolate and characterize yeast strains from selected traditional fermented foods of Manipur and Mizoram, India, and study their oleaginous attributes for biodiesel production. Fourteen potential oleaginous yeasts were isolated from fermented food samples of Manipur and Mizoram, India. The isolates were identified by 5.8S internal transcribed spacer (ITS) rRNA gene sequencing. Intracellular TAG accumulation by yeast cells were confirmed by Nile red fluorescence microscopy and spectrometry technique. The most promising isolates were evaluated for lipid accumulation having different initial carbon to nitrogen (C/N) ratios and also the full kinetic studies (depicting the glucose consumption, biomass, and lipid production) using optimum C/N ratio were estimated. Fatty acid methyl esters (FAME) profile of the transesterified lipids were analyzed by GC-MS. The identified yeast isolates belonged to seven different genera viz. Rhodotorula, Pichia, Candida, Saturnispora, Wickerhamomyces, Zygoascus, and Saccharomyces. Under nitrogen-limiting conditions, maximum biomass concentration of 5.66 ± 0.03 g/L and 4.713 ± 0.03 g/L was produced by Wickerhamomyces anomalus FK09 and Pichia kudriavzevii FK02, respectively. The highest lipid concentration (g lipid/L fermentation broth) of 0.58 g/L was attained by Rhodotorula mucilaginosa R2, followed by Wickerhamomyces anomalus FK09 (0.51 g/L), and Zygoascus hellenicus FC10 (0.41 g/L). Rhodotorula mucilaginosa R2 exhibited the maximum lipid content (% lipid/g dry cell weight) of (21.63 ± 0.1%) after 96 h of growth. The C/N ratio of 40 and 20 was found to be optimum for R. mucilaginosa R2 and W. anomalus FK09 with a lipid content of 22.21 ± 0.4% and 12.83 ± 0.08% respectively. Newly isolated yeast strains were obtained from traditional fermented food samples of Manipur and Mizoram, India. FAME analysis of the transesterified lipid extracts suggested the potential use of yeast-derived oil as an alternative to vegetable oil for biodiesel production.

中文翻译：

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从印度曼尼普尔邦和米佐拉姆邦的传统发酵食品和饮料中分离出的油质酵母，作为生物柴油生产中微生物脂质的有效来源

含油酵母可在营养限制条件下积聚细胞内脂质体或三酰基甘油酯（TAG）。从那些酵母菌株衍生的TAG被认为是用于生物柴油生产的常规植物油的替代物。在这项研究中，我们尝试从印度Manipur和Mizoram的精选传统发酵食品中分离和鉴定酵母菌株，并研究其对生物柴油生产的油脂特性。从印度曼尼普尔邦和米佐拉姆邦的发酵食品样品中分离出十四种潜在的含油酵母。通过5.8S内部转录间隔区（ITS）rRNA基因测序鉴定分离株。通过尼罗红荧光显微镜和光谱技术证实了酵母细胞在细胞内TAG的积累。评价了最有前途的分离物的脂质积累，这些脂质具有不同的初始碳氮比（C / N），并且还评估了使用最佳C / N比进行的完整动力学研究（描述了葡萄糖消耗，生物量和脂质产生）。通过GC-MS分析酯交换的脂质的脂肪酸甲酯（FAME）谱。鉴定出的酵母分离物属于七个不同属。红景天，毕赤酵母，假丝酵母​​，土生孢子，柳条霉菌，合酵母和酵母菌。在氮限制条件下，异常Wickerhamomyces FK09和Pichia kudriavzevii FK02分别产生最大生物量浓度为5.66±0.03 g / L和4.713±0.03 g / L。Rhodotorula mucilaginosa R2的最高脂质浓度（g脂质/ L发酵液）为0.58 g / L，紧随其后的是Wickerhamomyces anomalus FK09（0.51 g / L）和Zygoascus hellenicus FC10（0.41 g / L）。生长96小时后，粘质红假单胞菌R2表现出最大脂质含量（％脂质/ g干细胞重量）（21.63±0.1％）。发现C / N比为40和20时，分别对粘菌R2和异常W. FK09最佳，脂质含量分别为22.21±0.4％和12.83±0.08％。新分离的酵母菌株是从印度Manipur和Mizoram的传统发酵食品样品中获得的。对酯交换的脂质提取物进行的FAME分析表明，将酵母衍生的油作为植物油的替代品可用于生物柴油生产。生长96小时后1％）。发现C / N比为40和20时，分别对粘菌R.2和异常W.FK09的脂质含量分别为22.21±0.4％和12.83±0.08％最为合适。新分离的酵母菌株是从印度Manipur和Mizoram的传统发酵食品样品中获得的。对酯交换的脂质提取物进行的FAME分析表明，将酵母衍生的油作为植物油的替代品可用于生物柴油生产。生长96小时后1％）。发现C / N比为40和20时，分别对粘菌R.2和异常W.FK09的脂质含量分别为22.21±0.4％和12.83±0.08％最为合适。新分离的酵母菌株是从印度Manipur和Mizoram的传统发酵食品样品中获得的。对酯交换的脂质提取物进行的FAME分析表明，将酵母衍生的油作为植物油的替代品可用于生物柴油生产。