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Sonophotocatalysis-mediated morphological transition modulates virulence and antibiotic resistance in Salmonella Typhimurium
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0ew00224k A. P. Habeeb Rahman 1, 2, 3, 4, 5 , Ananyo Jyoti Misra 1, 2, 3, 4, 5 , Swagatika Panda 3, 4, 6 , Bhaskar Das 4, 7, 8, 9 , Prachi Bhol 1, 2, 3, 4 , Priti Sundar Mohanty 1, 2, 3, 4, 5 , Ashok J. Tamhankar 2, 3, 4, 5, 10 , Amrita Mishra 2, 3, 4, 5 , Cecilia Stålsby Lundborg 10, 11, 12, 13 , Suraj K. Tripathy 1, 2, 3, 4, 5
Environmental Science: Water Research & Technology ( IF 3.5 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0ew00224k A. P. Habeeb Rahman 1, 2, 3, 4, 5 , Ananyo Jyoti Misra 1, 2, 3, 4, 5 , Swagatika Panda 3, 4, 6 , Bhaskar Das 4, 7, 8, 9 , Prachi Bhol 1, 2, 3, 4 , Priti Sundar Mohanty 1, 2, 3, 4, 5 , Ashok J. Tamhankar 2, 3, 4, 5, 10 , Amrita Mishra 2, 3, 4, 5 , Cecilia Stålsby Lundborg 10, 11, 12, 13 , Suraj K. Tripathy 1, 2, 3, 4, 5
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Water resources contaminated with antibiotic-resistant (ABR) gastrointestinal pathogens pose severe health risks to society. Unfortunately, the limitations of traditional water treatment methodologies are leading to the evolution and dissemination of ABR microbes. In this aspect, a sonophotocatalytic (SPC) process is demonstrated for the successful disinfection of ABR Salmonella Typhimurium (STm) using Fe-doped ZnO nanoparticles (Fe–ZnO NPs) under visible-LED light. Reactive oxygen species (ROS) produced during SPC contributed to the detrimental effects on the microbes. In general, little is known regarding how bacteria modulate their phenotypic attributes during the course of disinfection. Our investigations to understand the effects of sublethal SPC on the expression of numerous phenotypic features of STm revealed changes in the virulence and ABR profiles. Sublethal SPC caused STm to adhere more on HCT116 cells, whereas their invasion properties were substantially reduced. The loss of ABR was recorded after sublethal SPC, although the resistance was partially regained after a nutrient source was provided to the bacteria. The manifestation of a morphological transition in STm from rod to cocci observed after sublethal SPC may have resulted in the significant loss of invasiveness and ABR. Further, SPC did not result in any process resistance; thus, it is a good candidate for water disinfection.
中文翻译：
声光催化介导的形态学转变调控鼠伤寒沙门氏菌的毒力和抗生素抗性
受到抗生素抗性（ABR）胃肠道病原体污染的水资源对社会构成了严重的健康风险。不幸的是，传统水处理方法的局限性导致了ABR微生物的进化和传播。在这方面，声光催化（SPC）工艺被证明可成功消毒ABR沙门氏菌在可见LED灯下使用掺铁的ZnO纳米颗粒（Fe-ZnO NPs）接种伤寒菌（STm）。SPC过程中产生的活性氧（ROS）会对微生物产生有害影响。通常，关于细菌在消毒过程中如何调节其表型属性的了解甚少。我们的研究了解亚致死性SPC对STm众多表型特征表达的影响，揭示了毒力和ABR谱的变化。亚致死性SPC导致STm更多地粘附在HCT116细胞上，而其侵袭特性却大大降低。在亚致死性SPC后记录了ABR的损失，尽管在向细菌提供营养源后抗性已部分恢复。在亚致死性SPC后观察到STm从杆向球菌的形态学转变可能导致浸润性和ABR的显着丧失。此外，SPC不会导致任何过程阻力。因此，它是水消毒的良好选择。
更新日期：2020-07-02
中文翻译：
声光催化介导的形态学转变调控鼠伤寒沙门氏菌的毒力和抗生素抗性
受到抗生素抗性（ABR）胃肠道病原体污染的水资源对社会构成了严重的健康风险。不幸的是，传统水处理方法的局限性导致了ABR微生物的进化和传播。在这方面，声光催化（SPC）工艺被证明可成功消毒ABR沙门氏菌在可见LED灯下使用掺铁的ZnO纳米颗粒（Fe-ZnO NPs）接种伤寒菌（STm）。SPC过程中产生的活性氧（ROS）会对微生物产生有害影响。通常，关于细菌在消毒过程中如何调节其表型属性的了解甚少。我们的研究了解亚致死性SPC对STm众多表型特征表达的影响，揭示了毒力和ABR谱的变化。亚致死性SPC导致STm更多地粘附在HCT116细胞上，而其侵袭特性却大大降低。在亚致死性SPC后记录了ABR的损失，尽管在向细菌提供营养源后抗性已部分恢复。在亚致死性SPC后观察到STm从杆向球菌的形态学转变可能导致浸润性和ABR的显着丧失。此外，SPC不会导致任何过程阻力。因此，它是水消毒的良好选择。
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