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Attenuated Salmonella typhimurium-mediated tumour targeting imaging based on peptides.
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0bm00013b Saisai Xiong 1 , Zhongyuan Qi , Jing Ni , Jing Zhong , Lijuan Cao , Kai Yang
Biomaterials Science ( IF 5.8 ) Pub Date : 2020-05-14 , DOI: 10.1039/d0bm00013b Saisai Xiong 1 , Zhongyuan Qi , Jing Ni , Jing Zhong , Lijuan Cao , Kai Yang
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Attenuated bacteria-mediated tumor targeting diagnosis will provide a novel strategy for further cancer treatments owing to the intrinsic facultative anaerobic characteristic of bacteria and rapid proliferation in the tumor sites. In this work, we firstly investigate the in vivo behaviour of the attenuated Salmonella typhimurium (S. typhimurium ΔppGpp/lux) after intravenous injection. S. typhimurium exhibits rapid proliferation in tumor sites after three days of injection through bioluminescence imaging, the Luria–Bertani plate and the Gram-staining assay. In contrast, S. typhimurium does not proliferate in the normal tissues and could be excreted from the body of mice. Afterwards, a targeting peptide ubiquicidin (UBI) labeled with fluorescent dye Cy5.5 or radionuclide 125I was intravenously injected into the mice with or without S. typhimurium treatments for in vivo fluorescence imaging and single-photon emission computed tomography (SPECT/CT) imaging, respectively. The results show that the peptide UBI could specifically target the two independent bacteria-infected tumor models, the 4T1 murine breast cancer model and the CT26 mouse colon cancer model, realizing the sensitive multimodal imaging of tumors. Such a strategy (bacteria-mediated tumor targeting) may further improve the sensitivity to early diagnosis of tumors. We hope that our developed strategy could further be extended to cancer theranostics in the future, bringing good news for cancer patients.
中文翻译：
基于肽的减毒鼠伤寒沙门氏菌介导的肿瘤靶向成像。
由于细菌固有的兼性厌氧特性和肿瘤部位的快速增殖，减毒的细菌介导的肿瘤靶向诊断将为进一步的癌症治疗提供新的策略。在这项工作中，我们首先研究了静脉注射后减毒鼠伤寒沙门氏菌（鼠伤寒沙门氏菌ΔppGpp/ lux）的体内行为。通过生物发光成像，Luria-Bertani平板和革兰氏染色法注射三天后鼠伤寒沙门氏菌在肿瘤部位迅速增殖。相反，鼠伤寒沙门氏菌不会在正常组织中增殖，并可能从小鼠体内排出。然后，用荧光染料标记Cy5.5的或放射性核素靶向肽ubiquicidin（UBI）125我静脉内注射到小鼠中有或没有鼠伤寒沙门氏菌治疗为在体内荧光成像和单光子发射计算机断层扫描（SPECT / CT）成像。结果表明，该肽UBI可以特异性靶向两种独立的细菌感染的肿瘤模型，即4T1鼠乳腺癌模型和CT26小鼠结肠癌模型，实现了敏感的肿瘤多模态成像。这种策略（细菌介导的肿瘤靶向）可以进一步提高对肿瘤早期诊断的敏感性。我们希望将来我们开发的策略可以进一步扩展到癌症治疗学，为癌症患者带来好消息。
更新日期：2020-06-30
中文翻译：
基于肽的减毒鼠伤寒沙门氏菌介导的肿瘤靶向成像。
由于细菌固有的兼性厌氧特性和肿瘤部位的快速增殖，减毒的细菌介导的肿瘤靶向诊断将为进一步的癌症治疗提供新的策略。在这项工作中，我们首先研究了静脉注射后减毒鼠伤寒沙门氏菌（鼠伤寒沙门氏菌ΔppGpp/ lux）的体内行为。通过生物发光成像，Luria-Bertani平板和革兰氏染色法注射三天后鼠伤寒沙门氏菌在肿瘤部位迅速增殖。相反，鼠伤寒沙门氏菌不会在正常组织中增殖，并可能从小鼠体内排出。然后，用荧光染料标记Cy5.5的或放射性核素靶向肽ubiquicidin（UBI）125我静脉内注射到小鼠中有或没有鼠伤寒沙门氏菌治疗为在体内荧光成像和单光子发射计算机断层扫描（SPECT / CT）成像。结果表明，该肽UBI可以特异性靶向两种独立的细菌感染的肿瘤模型，即4T1鼠乳腺癌模型和CT26小鼠结肠癌模型，实现了敏感的肿瘤多模态成像。这种策略（细菌介导的肿瘤靶向）可以进一步提高对肿瘤早期诊断的敏感性。我们希望将来我们开发的策略可以进一步扩展到癌症治疗学，为癌症患者带来好消息。
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