Lung cancer remains one of the leading causes of cancer deaths around the world. Previous studies have shown that microRNAs have pivotal functions in tumorigenesis including lung cancer. It is reported that microRNA-195-5p acts as a tumor suppressor role in human cancers. However, the function and molecular mechanism of microRNA-195-5p in lung cancer progression is still unclear. In the present study, the results showed that the expression of microRNA-195-5p was downregulated both in lung cancer tissues and in lung cancer cell lines. Enhanced expression of microRNA-195-5p inhibited cell proliferation, migration, and invasion in lung cancer cells. Furthermore, Forkhead box k1 was identified as the direct target of microRNA-195-5p. Forkhead box k1 overexpression could restore the repressed cell proliferation and metastasis caused by microRNA-195-5p overexpression. Our results demonstrated that a functional mechanism of microRNA-195-5p in regulating lung cancer. It indicates that microRNA-195-5p may regulate lung cancer growth and metastasis through the regulation of Forkhead box k1, highlighting the potential application for the treatment of lung cancer in the future.

中文翻译：

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miR-195-5p通过FOXK1抑制肺癌细胞的增殖，迁移和侵袭。

肺癌仍然是全世界癌症死亡的主要原因之一。先前的研究表明，microRNA在包括肺癌在内的肿瘤发生中具有关键作用。据报道，microRNA-195-5p在人类癌症中起着抑癌作用。然而，microRNA-195-5p在肺癌进展中的功能和分子机制仍不清楚。在本研究中，结果表明在肺癌组织和肺癌细胞系中，microRNA-195-5p的表达均被下调。microRNA-195-5p的增强表达抑制肺癌细胞中的细胞增殖，迁移和侵袭。此外，叉头盒k1被确定为microRNA-195-5p的直接靶标。叉头箱k1过表达可恢复因microRNA-195-5p过表达引起的抑制的细胞增殖和转移。我们的结果证明了microRNA-195-5p在调节肺癌中的功能机制。这表明microRNA-195-5p可能通过调节叉头盒k1来调节肺癌的生长和转移，突出了将来在治疗肺癌中的潜在应用。