The ability of white-rot fungus, Trametes hirsuta AK04, to utilize phenolics as single and mixed substrates was determined in mineral medium and palm oil mill effluent (POME). The strain AK04 was able to rapidly metabolize all ten phenolics as single and mixed substrates at all test concentrations. With single substrates, between 78 and 98% removal was achieved within seven days. The biomass yield increased with increasing concentration from 100 to 500 mg L⁻¹ but slightly decreased when the concentration was increased up to 1,000 mg L⁻¹. When fitted to a Haldane model, the groups of benzoic and cinnamic acid derivatives gave significantly higher maximum specific growth rates than other phenolics. Phenol exhibited the lowest affinity and highest inhibitory effects on fungal metabolism. In mixed substrates, the total concentration ranges of phenolics mixtures between 1,000 and 6,000 mg L⁻¹ did not affect the fungal growth rate and the strain AK04 showed a high degree of resistance to their toxic effects. The addition of glucose and yeast extract enhanced the degradation rates of individual phenolics in the substrate mixtures, demonstrating the advantage of this strain for treating complex media, such as industrial wastewater.

在矿物培养基和棕榈油厂废水（POME）中确定了白腐真菌Trametes hirsuta AK04利用酚类作为单一底物和混合底物的能力。AK04菌株能够在所有测试浓度下快速代谢所有十种酚类化合物，作为单一底物和混合底物。使用单一基材，在7天内可实现78％至98％的去除率。随着浓度从100到500 mg L ^-1增加，生物量产量增加，但是当浓度增加到1,000 mg L ^-1时，生物量产量略有下降。。当与Haldane模型拟合时，苯甲酸和肉桂酸衍生物的组比其他酚类化合物具有更高的最大比生长速率。苯酚对真菌代谢表现出最低的亲和力和最高的抑制作用。在混合底物中，酚类混合物的总浓度范围在1,000至6,000 mg L ^-1之间，不会影响真菌的生长速率，并且AK04菌株对它们的毒性作用表现出高度的抵抗力。葡萄糖和酵母提取物的添加提高了底物混合物中单个酚类的降解速度，这表明该菌株具有处理复杂介质（例如工业废水）的优势。