Introduction

The American cockroach (Periplaneta americana) is used in traditional Chinese medicine. Periplaneta americana (P. americana) is rich in oil that has shown potential antioxidant and antibacterial activities in vitro.

Objective

To evaluate the safety of oil extracted from P. americana by conducting acute dermal toxicity, irritation, and sensitization tests.

Materials and methods

In an acute dermal toxicity study, adult Sprague–Dawley rats were exposed to P. americana oil (2000 mg/kg body weight) for 24 h. Clinical observations were conducted to evaluate the toxicity, behaviour, and health of the animals every day after dermal exposure for 14 days. For the dermal irritation test, the oil was applied to rabbits in single and multiple doses. Multi-dose treatment was administered once per day for 14 days. Each rabbit served as its own left- and right-side control and the rabbits’ irritation reactions in local intact and damaged skin were recorded and scored. The skin sensitization study of guinea pigs with the oil was conducted for a period of 28 days.

Results

The acute dermal median lethal dose (LD₅₀) of P. americana oil was > 2000 mg/kg body weight in adult rats. There was no significant difference in mean irritation scores between the negative control and oil groups. The oil caused very little or no irritation in the intact and damaged skin rabbits treated with either single or multiple doses and it was non-sensitizing to the skin of guinea pigs.

Conclusions

These results suggest that P. americana oil does not produce any significant acute toxic effects and is safe for use in animal models with almost no dermal irritation or sensitization. Therefore, it presents a low risk of provoking skin reactions in humans.

中文翻译：

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从美洲大plane提取的油的皮肤安全性评估：急性皮肤毒性，刺激性和致敏性。

介绍

美国蟑螂（Periplaneta americana）用于传统中药。美洲大plane（P. americana）富含石油，在体外显示出潜在的抗氧化和抗菌活性。

目的

评估从P中提取的油的安全性。通过进行急性皮肤毒性，刺激性和致敏性试验进行美洲试验。

材料和方法

在急性皮肤毒性研究，成年SD大鼠暴露于P。美国油（2000毫克/千克体重）持续24小时。皮肤暴露14天后，每天进行临床观察以评估动物的毒性，行为和健康状况。对于皮肤刺激试验，将油以单剂量和多剂量施用于兔子。每天一次进行多剂量治疗，持续14天。每只兔子用作其自己的左侧和右侧对照，并记录兔子的局部完整和受损皮肤的刺激反应并进行评分。用油对豚鼠的皮肤致敏研究进行了28天。

结果

P的急性皮肤中位致死剂量（LD ₅₀）。成年大鼠中美洲油的含量> 2000 mg / kg体重。阴性对照组和油组之间的平均刺激评分没有显着差异。该油对用单次或多次剂量处理的完整和受损皮肤兔子的刺激很小或没有刺激，并且对豚鼠的皮肤不敏感。

结论

这些结果表明，P。美洲油不会产生任何明显的急性毒性作用，可安全用于几乎没有皮肤刺激或致敏作用的动物模型。因此，它引起人类皮肤反应的风险较低。