Abstract This research investigated the effect of ozonized rapeseed oil on the bioremediation of soil contaminated with diesel oil hydrocarbons performed by Bacillus mycoides NS1020. It is a new approach to cleaning up soil contaminated with petroleum compounds combining chemical and biological remediation. Two different doses of ozone (0.5 mg O3g−1 hydrocarbons, 2 mgO3g−1 hydrocarbons) and three different doses of rapeseed oil (10 g kg−1 soil, 50 g kg−1 soil, 100 g kg1 soil) were used. During the process, the contamination reduction, changes in soil dehydrogenase activity and pH were monitored. After 8 weeks of bioremediation, the loss of hydrocarbons in samples enhanced with rapeseed oil and ozonized rapeseed oil ranged between 97 and 99.7%. An inhibition in the metabolic activity of microorganisms (from 6 to 95%) in the first stage in trials enhanced with ozonized rapeseed oil was observed while non-ozonized vegetable oil increased the values of soil dehydrogenase activity. All the results indicated that a mixed treatment including ozonized vegetable oil and microbial degradation is a promising bioremediation technology for cleaning up soil contaminated with diesel fuel.

中文翻译：

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臭氧化菜籽油对芽孢杆菌NS1020柴油污染土壤的生物修复作用

摘要 本研究调查了臭氧化菜籽油对由芽孢杆菌 NS1020 进行的柴油烃污染土壤的生物修复作用。它是一种结合化学和生物修复的清理被石油化合物污染的土壤的新方法。使用了两种不同剂量的臭氧（0.5 mg O3g-1 碳氢化合物、2 mgO3g-1 碳氢化合物）和三种不同剂量的菜籽油（10 g kg-1 土壤、50 g kg-1 土壤、100 g kg-1 土壤）。在此过程中，监测污染减少、土壤脱氢酶活性和 pH 值的变化。经过 8 周的生物修复，菜籽油和臭氧化菜籽油增强的样品中碳氢化合物的损失范围在 97% 到 99.7% 之间。在用臭氧化菜籽油增强的试验的第一阶段观察到微生物代谢活性的抑制（从 6% 到 95%），而未臭氧化的植物油增加了土壤脱氢酶活性的值。所有结果表明，臭氧化植物油和微生物降解的混合处理是一种很有前景的生物修复技术，用于清理被柴油污染的土壤。