Abstract The present investigation deal with study of chemical composition of Harsingar (Nyctanthes arbortristis) essential oil from leaves antimicrobial and antioxidant potential of essential oil, major compound eugenol and its derivatives. The essential oil was extracted through hydro-distillation using Clevenger apparatus. Gas chromatography-Mass spectrometry analysis of essential oil revealed the presence of eugenol (88.20 %) as major compound which was isolated from essential oil by column chromatography. It was chemically transformed into two derivatives i.e. eugenol -5- aldehyde (2) and 5-allyl-2-hydroxy-3-methoxybenzenesulfonic acid (3). The essential oil, eugenol and its derivatives were evaluated for their antimicrobial activity against Klebsiella pneumoniae and Pseudomonas aeruginosa by disc plate method and against Fusarium oxysporum using agar well diffusion method. They were also evaluated for their antioxidant potential by 2, 2-diphenyl-1- picrylhydrazyl (DPPH) and NO free radical scavenging activities. The essential oil showed maximum antibacterial potential with diameter of inhibition zone of 23.8 and 26.3 mm at 1000 μg/ml against K. pneumoniae and P. aeruginosa respectively. 5-allyl-2-hydroxy-3-methoxybenzenesulfonic acid (3) was most effective against F. oxysporum with maximum diameter of inhibition zone of 29.5 mm at 2500 μg/ml. Eugenol (1) showed maximum antioxidant potential with 90.77 and 88.99 % radical scavenging in DPPH and NO radical scavenging assays having IC50 values of 18.07 and 26.50 μg/ml respectively. Harsingar essential oil, eugenol and its derivatives may be used as promising antimicrobial and antioxidising agents and will help in the development of natural antimicrobials and antioxidants as substitute for their synthetic analogues.

中文翻译：

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Harsingar (Nyctanthes arbortristis) 精油的化学分析、抗菌和抗氧化活性

摘要 本研究涉及从叶子中提取的 Harsingar (Nyctanthes arbortristis) 精油的化学成分研究，精油、主要化合物丁香酚及其衍生物的抗菌和抗氧化潜力。使用 Clevenger 装置通过水蒸馏提取精油。精油的气相色谱-质谱分析表明存在丁香酚 (88.20%) 作为主要化合物，通过柱色谱从精油中分离出来。它被化学转化为两种衍生物，即丁子香酚-5-醛 (2) 和 5-烯丙基-2-羟基-3-甲氧基苯磺酸 (3)。精油，丁香酚及其衍生物通过圆盘法评估了它们对肺炎克雷伯菌和铜绿假单胞菌的抗菌活性，并使用琼脂井扩散法对尖孢镰刀菌进行了评估。还通过 2, 2-二苯基-1-苦基肼 (DPPH) 和 NO 自由基清除活性评估了它们的抗氧化潜力。该精油在 1000 μg/ml 时对肺炎克雷伯菌和铜绿假单胞菌显示出最大的抗菌潜力，抑菌圈直径分别为 23.8 和 26.3 毫米。5-烯丙基-2-羟基-3-甲氧基苯磺酸 (3) 对尖孢镰刀菌最有效，2500 μg/ml 时的最大抑菌圈直径为 29.5 mm。丁香酚 (1) 在 DPPH 和 NO 自由基清除试验中显示出最大的抗氧化潜力，其自由基清除率为 90.77 % 和 88.99 %，IC50 值为 18。分别为 07 和 26.50 μg/ml。Harsingar 精油、丁子香酚及其衍生物可用作有前景的抗微生物剂和抗氧化剂，并将有助于开发天然抗微生物剂和抗氧化剂作为其合成类似物的替代品。