Abstract

Three previously undescribed ent-abietane-type diterpenoids, designated as 11β-hydroxy-14-oxo-17-al-ent-abieta-8(9),13(15)-dien-16,12β-olide (1), 11β,17-dihydroxy-12-methoxy-ent-abieta-8(14),13(15)-dien-16,12ɑ-olide (2), and 14ɑ-hydroxy-17-al-ent-abieta-7(8),11(12),13(15)-trien-16,12-olide (3), were isolated from 95% ethanol extract of the roots of Euphorbia wallichii. The structures of the new compounds were elucidated by spectroscopic analysis (NMR, IR, UV, and MS). The isolated diterpenoids were tested in vitro for antimicrobial potentials against 6 pathogenic microorganisms. As a result, compounds 1-3 exhibited some antimicrobial activity against the tested Gram positive bacteria with minimum inhibitory concentration values less than 60 μg/ml.

摘要

三个以前未描述ENT -abietane型二萜，被指定为11 β -羟基-14-氧代-17- AL- ENT -abieta-8（9），13（15） -二烯16,12 β -olide（1） ，11 β，17二羟基-12-甲氧基ENT -abieta-8（14），13（15） -二烯16,12 ɑ -olide（2），和14 ɑ羟基-17-AL- ENT - abieta-7(8),11(12),13(15)-trien-16,12-olide ( 3 ), 是从大戟根的 95% 乙醇提取物中分离出来的. 通过光谱分析（NMR、IR、UV和MS）阐明了新化合物的结构。在体外测试了分离的二萜类化合物对 6 种病原微生物的抗菌潜力。其结果，化合物1 - 3表现出一些抗微生物活性测试的革兰氏阳性细菌用最小抑制浓度值小于60微克/毫升。