We previously reported that following long-term hypoxia (LTH), the ovine foetus exhibits enhanced expression of brown/beige adipose genes. This study was designed to determine if these changes are preserved after birth. Pregnant ewes were divided among three groups, 1) Control, sea level, 2) LTH, high altitude (3,820 m, LTH-HA) from ~ day 40 of gestation through ~14 days post-delivery and 3) LTH from ⁓ day 40 through day 137 of gestation then returned to the laboratory where atory reduced maternal PO2 was maintained by nitrogen infusion. Following delivery, lambs remained at sea level (LTH-SL). Perirenal adipose tissue was collected at ~day 14, and qRT-PCR was used to quantify mRNA. Uncoupling protein 1 (UCP-1), PPAR gamma coactivator 1 (PGC1α), and deiodinase-2 (DIO2) mRNA levels were significantly lower in both LTH groups while PR domain containing 16 (PRDM16) levels did not differ. Peroxisome proliferator-activated receptor (PPARγ) was maintained in the LTH-HA group and significantly increased in the LTH-SL group, compared to control. Unlike our previous LTH foetal studies, the brown/beige fat phenotype was rapidly lost by day 14 postpartum compared to control, while PPARγ was maintained. This loss of the brown fat phenotype may promote obesity due to decreased energy expenditure, favouring fat deposition.

中文翻译：

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妊娠期间长期缺氧会改变羔羊的肾周脂肪组织基因表达。

我们之前曾报道，在长期缺氧 (LTH) 后，绵羊胎儿表现出棕色/米色脂肪基因的增强表达。本研究旨在确定这些变化是否在出生后得以保留。怀孕的母羊被分为三组，1) 对照组，海平面，2) LTH，高海拔（3,820 米，LTH-HA），从妊娠第 40 天到分娩后第 14 天，以及 3）从第 40 天开始的 LTH到妊娠第 137 天，然后返回实验室，通过输注氮气维持降低的母体 PO2。交付后，羔羊仍处于海平面 (LTH-SL)。在第 14 天收集肾周脂肪组织，并使用 qRT-PCR 对 mRNA 进行定量。解偶联蛋白 1 (UCP-1)、PPAR γ 辅激活因子 1 (PGC1α)、和脱碘酶-2 (DIO2) mRNA 水平在两个 LTH 组中均显着降低，而含有 16 (PRDM16) 的 PR 结构域水平没有差异。与对照组相比，过氧化物酶体增殖物激活受体 (PPARγ) 在 LTH-HA 组中得到维持，在 LTH-SL 组中显着增加。与我们之前的 LTH 胎儿研究不同，与对照组相比，棕色/米色脂肪表型在产后第 14 天迅速消失，而 PPARγ 得以维持。由于能量消耗减少，有利于脂肪沉积，这种棕色脂肪表型的丧失可能会促进肥胖。与对照相比，到产后第 14 天，棕色/米色脂肪表型迅速消失，而 PPARγ 得以维持。由于能量消耗减少，有利于脂肪沉积，这种棕色脂肪表型的丧失可能会促进肥胖。与对照相比，到产后第 14 天，棕色/米色脂肪表型迅速消失，而 PPARγ 得以维持。由于能量消耗减少，有利于脂肪沉积，这种棕色脂肪表型的丧失可能会促进肥胖。