The outer membrane protein of Aeromonas hydrophila is a potential candidate for vaccine development. In this study, after cloning and expression of ompTS, 270 common carp, weighing 44 ± 5.7 g divided into five groups, were injected intraperitoneally twice with 3‐week intervals. Groups included the following: PBS, PBS plus Freund's adjuvant, recombinant protein, recombinant protein plus Freund's adjuvant and 20 fish as negative control. Two weeks after the second injection, 30 fish of each group were challenged with a dose of 2 × LD50 of Aeromonas hydrophila and RPS was measured. The antibody level was measured using ELISA test. The protection of recombinant protein in the immunized fish with and without adjuvant, respectively, was about 82.61% and 78.26% (the protection of recombinant protein electroeluted from an SDS–PAGE with and without adjuvant, respectively, was about 78.62% and 69.57%). The average of antibody level in recombinant protein with and without adjuvant was significantly higher than the PBS group (p < .05). The ability of recombinant ompTS to increase the antibody level and to protect the fish from challenge by A. hydrophila demonstrated that recombinant ompTS protein injection can be used to immunize common carp against A. hydrophila infection.

中文翻译：

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嗜水气单胞菌重组蛋白（OmpTS）对鲤鱼（Cyprinus carpio）保护作用的研究

嗜水气单胞菌的外膜蛋白是疫苗开发的潜在候选者。在这项研究中，在克隆和表达 ompTS 后，将 270 条重 44 ± 5.7 g 的鲤鱼分成 5 组，以 3 周的间隔腹腔注射两次。组包括以下：PBS、PBS加弗氏佐剂、重组蛋白、重组蛋白加弗氏佐剂和20条鱼作为阴性对照。第二次注射后两周，每组 30 条鱼接受剂量为 2 × LD50 的嗜水气单胞菌并测量 RPS。使用ELISA测试测量抗体水平。有佐剂和无佐剂的免疫鱼中重组蛋白的保护率分别约为82.61%和78%。26%（含有和不含佐剂的 SDS-PAGE 电洗脱重组蛋白的保护率分别约为 78.62% 和 69.57%）。含佐剂和不含佐剂的重组蛋白中抗体水平的平均值显着高于 PBS 组 (p < .05)。重组 ompTS 提高抗体水平和保护鱼类免受嗜水 A. 攻击的能力表明，重组 ompTS 蛋白注射液可用于免疫鲤鱼对抗嗜水 A. 感染。