Histoplasma capsulatum is a dimorphic fungus that most frequently causes pneumonia, but can also disseminate and proliferate in diverse tissues. Histoplasma capsulatum has a complex secretion system that mediates the release of macromolecule‐degrading enzymes and virulence factors. The formation and release of extracellular vesicles (EVs) are an important mechanism for non‐conventional secretion in both ascomycetes and basidiomycetes. Histoplasma capsulatum EVs contain diverse proteins associated with virulence and are immunologically active. Despite the growing knowledge of EVs from H. capsulatum and other pathogenic fungi, the extent that changes in the environment impact the sorting of organic molecules in EVs has not been investigated. In this study, we cultivated H. capsulatum with distinct culture media to investigate the potential plasticity in EV loading in response to differences in nutrition. Our findings reveal that nutrition plays an important role in EV loading and formation, which may translate into differences in biological activities of these fungi in various fluids and tissues.

中文翻译：

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媒体很重要！响应不同营养环境的荚膜组织胞浆菌细胞外囊泡的加载和释放的改变。

荚膜组织胞浆菌是一种二形性真菌，最常引起肺炎，但也可以在不同组织中传播和增殖。荚膜组织胞浆菌具有复杂的分泌系统，可介导大分子降解酶和毒力因子的释放。细胞外囊泡（EVs）的形成和释放是子囊菌和担子菌非常规分泌的重要机制。荚膜组织胞浆菌EV 含有多种与毒力相关的蛋白质，并且具有免疫活性。尽管越来越多地了解来自H. capsulatum的 EV和其他病原真菌，环境变化对电动汽车中有机分子分类的影响程度尚未得到研究。在这项研究中，我们用不同的培养基培养了荚膜梭状芽孢杆菌，以研究 EV 负载对营养差异的潜在可塑性。我们的研究结果表明，营养在 EV 负载和形成中起着重要作用，这可能会转化为这些真菌在各种体液和组织中的生物活性差异。