Abstract As a powerful technique, gene transformation can produce new lily cultivars with specific characteristics such as new flower colors and resistance to pathogens. However, it is difficult to use this technique in monocot plants like lilies. A new phenolic compound called ‘chloroxynil’ has been introduced to replace ‘acetosyringone’ and is considered to improve transformation efficiency of Lilium. Our results showed that the addition of chloroxynil into the inoculation and co-cultivation media significantly improved the transformation efficiency. At 4 μM, chloroxynil-mediated callus regeneration increased 7 days after it was transferred to the regeneration medium. Transformation efficiency was 6.6 % at 100 μM acetosyringone, while 11.1 % at 4 μM CX. Finally, 11 kanamycin-resistant plants were successfully produced at 4 μM CX, and gene transformation in the plantlets was validated by PCR and RT-PCR techniques.

中文翻译：

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'氯苯腈'对农杆菌介导的百合'Manissa'转化效率的影响

摘要 作为一项强大的技术，基因转化可以产生具有特定特征的新百合品种，例如新的花色和对病原体的抗性。然而，这种技术很难在百合等单子叶植物中使用。一种名为“氯苯乙腈”的新型酚类化合物已被引入以取代“乙酰丁香酮”，并被认为可以提高百合的转化效率。我们的结果表明，在接种和共培养培养基中加入氯苯腈显着提高了转化效率。在 4 μM 时，氯苯腈介导的愈伤组织再生在将其转移到再生培养基后 7 天增加。转化效率在 100 μM 乙酰丁香酮时为 6.6%，而在 4 μM C​​X 时为 11.1%。最后，在 4 μM C​​X 下成功生产了 11 株卡那霉素抗性植物，