The aim of plant gene banks is to preserve genetic resources selected based on their phenotypic, agronomic, historical or other cultural values for future utilization. In the present study the modified PVS2 droplet vitrification technique was tested and optimized for cryopreservation of a large diversity of blackcurrant (R. nigrum L.) accessions propagated in vitro and selected into a national gene bank core collection. Out of four accessions tested to optimize the method, three recovered and regenerated by 89–97% on average, but one recalcitrant in vitro line only by 25%. The tested post-cryopreservation recovery media with different macronutrient and growth regulator levels showed no generalized effect on regenerated shoots, but the effect of recovery media was different between cultivars. When the whole regeneration chain from cryopreservation via micropropagation to greenhouse conditions was tested, shoots at least 1 cm in length were found necessary for successful transfer ex vitro. The long-term cryopreservation of 22 blackcurrant accessions was finally conducted, with practices slightly modified from the tested protocol. The estimated recovery of shoot tips after 9 weeks in vitro was 17–94% with at least 75% recovery in seven accessions and at least 40% recovery in 19 out of 22 accessions. Only one accession had no cryopreservation success. The results demonstrated that the modified droplet vitrification technique is appropriate for a large diversity of blackcurrant accessions. However, cultivar-related differences and recovery procedures are to be considered for success in regeneration and ex vitro adaptation.

植物基因库的目的是保存根据表型，农艺，历史或其他文化价值选择的遗传资源，以备将来使用。在本研究中，对改良的PVS2液滴玻璃化技术进行了测试和优化，以用于冷冻保存大量的黑加仑（R. nigrum）L.）种质在体外繁殖，并入选为国家基因库核心收藏。在为优化该方法而测试的四份种质中，三份的平均回收率和再生率平均为89–97％，而一株顽固的体外品系的回收率仅为25％。经测试的低温保存后具有不同的常量营养素和生长调节剂水平的恢复培养基对再生芽没有普遍影响，但不同品种的恢复培养基的作用不同。测试了从低温保存到微繁殖到温室条件的整个再生链时，发现至少1厘米长的芽对成功地离体转移是必需的。最终进行了22种黑加仑子的长期冷冻保存，并从测试方案中稍作修改。在体外9周后，估计的茎尖恢复率为17–94％，其中7个入种的至少75％的恢复，以及22个入种的19个的至少40％的恢复。只有一种加入没有冷冻保存成功。结果表明，改进的液滴玻璃化技术适用于多种黑加仑品种。然而，与品种相关的差异和恢复程序将被考虑用于成功再生和离体适应。