Electroporation is a bioelectric phenomenon used to deliver target molecules into cells in vitro and irreversible electroporation (IRE) is an emerging cancer therapy used to treat inoperable tumors in situ. These phenomena are generally considered to be non-thermal in nature. In this study, a 3D tumor model was used to investigate the correlation between temperature and the effectiveness of standard clinical IRE and high frequency (H-FIRE) protocols. It was found for human glioblastoma cells that in the range of 2 to 37 °C the H-FIRE lethal electric field threshold value, which describes the minimum electric field to cause cell death, is highly dependent on temperature. Increasing the initial temperature from 2 to 37 °C resulted in a significant decrease in lethal electric field threshold from 1168 to 507 V/cm and a 139% increase in ablation size for H-FIRE burst treatments. Standard clinical protocol IRE treatments resulted in a decrease in lethal threshold from 485 to 453 V/cm and a 7% increase in ablation size over the same temperature range. Similar results were found for pancreatic cancer cells which indicate that tissue temperature may be a significant factor affecting H-FIRE ablation size and treatment planning in vivo while lower temperatures may be useful in maintaining cell viability for transfection applications.

中文翻译：

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在3D肿瘤模型中评估高频不可逆电穿孔的温度依赖性。

电穿孔是一种生物电现象，用于在体外将靶分子递送到细胞中，不可逆电穿孔（IRE）是一种新兴的癌症疗法，用于原位治疗无法手术的肿瘤。这些现象通常被认为是非热的。在这项研究中，使用3D肿瘤模型研究温度与标准临床IRE和高频（H-FIRE）方案的有效性之间的相关性。对于人类胶质母细胞瘤细胞，发现在2至37°C的范围内，H-FIRE致死电场阈值高度依赖于温度，该阈值描述了导致细胞死亡的最小电场。将初始温度从2°C升高到37°C，导致致命电场阈值从1168 V / cm显着降低到H-FIRE爆裂治疗，烧蚀尺寸增加139％。在相同温度范围内，标准的临床方案IRE治疗导致致死阈值从485 V / cm降低到453 V / cm，消融尺寸增加了7％。对于胰腺癌细胞发现了相似的结果，这表明组织温度可能是影响H-FIRE消融大小和体内治疗计划的重要因素，而较低的温度可能对于维持转染应用的细胞生存力有用。在相同温度范围内，标准的临床方案IRE治疗导致致死阈值从485 V / cm降低到453 V / cm，消融尺寸增加了7％。对于胰腺癌细胞发现了相似的结果，这表明组织温度可能是影响H-FIRE消融大小和体内治疗计划的重要因素，而较低的温度可能对于维持转染应用的细胞生存力有用。在相同温度范围内，标准的临床方案IRE治疗导致致死阈值从485 V / cm降低到453 V / cm，消融尺寸增加了7％。对于胰腺癌细胞发现了相似的结果，这表明组织温度可能是影响H-FIRE消融大小和体内治疗计划的重要因素，而较低的温度可能对于维持转染应用的细胞生存力有用。