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Biodiversity, spoilage capacity and heat resistance of mesophilic aerobic spores isolated from milk powders marketed in Algeria
International Journal of Dairy Technology ( IF 4.4 ) Pub Date : 2020-05-13 , DOI: 10.1111/1471-0307.12715
Meryem Benahmed 1, 2 , Ivan Leguerinel 3 , Boumedine Moussa‐Boudjemaa 1
Affiliation  

An analysis of milk powder isolates based on RAPD‐M13 and 16SrRNA sequencing has been conducted. Phylogenetic groups were distinguished by panC gene sequencing. An evaluation of the enzymatic activity and heat resistance of representative isolates has also been made. Molecular analysis revealed 17 groups. Bacillus cereus groups II, III and IV were the main phylogenetic groups. Bacillus cereus and B. subtilis groups demonstrated high proteolytic activity along with D95°C values ranging from 1 to 9 min and z‐values between 6.5 and 18.6 °C.

中文翻译:

从阿尔及利亚销售的奶粉中分离出的嗜温好氧孢子的生物多样性,变质能力和耐热性

进行了基于RAPD-M13和16SrRNA测序的奶粉分离株分析。系统发育基团通过panC基因测序进行区分。还对代表性分离物的酶活性和耐热性进行了评估。分子分析揭示了17个组。蜡状芽孢杆菌组II,III和IV是主要的系统发育组。蜡状芽孢杆菌枯草芽孢杆菌组表现出高蛋白水解活性,D 95°C值介于1至9分钟之间,z值介于6.5和18.6°C之间。
更新日期:2020-05-13
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