ABSTRACT

Classical macroautophagy/autophagy functions to maintain cell health during stressful conditions by targeting cytosolic components for degradation and recycling through the lysosomal pathway. In contrast, nondegradative secretory autophagy functions as an alternative autophagy mechanism to mediate extracellular secretion. A recent study published in Nature Cell Biology from the laboratory of Jayanta Debnath has identified components of the LC3-conjugation machinery as mediators in the selection of cargo for nonclassical secretion. Termed LC3-dependent extracellular vesicle loading and secretion (LDELS), this mechanism provides an additional link between secretory autophagy and the release of extracellular vesicles.

Abbreviations

ATG, autophagy-related; BioID, proximity-dependent biotinylation; CM, conditioned medium; EV, extracellular vesicle; HNRNPK, heterogeneous nuclear ribonuclear protein K; ILVs, intralumenal vesicles; LDELS, LC3-dependent EV loading and secretion; LIR, LC3-interacting region; MAP1LC3/LC3, microtubule associated protein 1 light chain 3; MS, mass spectrometry; MVBs, multivesicular bodies; ncRNA, non-coding RNA; NSMAF/FAN, neutral sphingomyelinase activation associated factor; P-bodies, processing bodies; PE, phosphatidylethanolamine; RB1CC1/FIP200, RB1 inducible coiled-coil 1; RBP, RNA-binding protein; RNA-seq, RNA sequencing; SAFB, scaffold-attachment factor B; SILAC, stable isotope labeling of amino acids in cell culture; SMPD3/nSMase2, sphingomyelin phosphodiesterase 3; TEM, transmission electron microscopy; TMT, tandem mass tagging

中文翻译：

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LC3偶联机制指定了货物装载和细胞外囊泡的分泌。

摘要

经典的巨噬细胞自噬/自噬的功能是通过靶向细胞溶质组分以通过溶酶体途径进行降解和再循环，从而在应激状态下维持细胞健康。相反，非降解性分泌自噬作用是介导细胞外分泌的另一种自噬机制。Jayanta Debnath实验室发表在《自然细胞生物学》上的一项最新研究已经确定，LC3缀合机制的成分可以作为选择非经典分泌物的媒介。这种机制被称为依赖LC3的细胞外小泡负载和分泌（LDELS），在分泌自噬与细胞外小泡释放之间提供了额外的联系。

缩略语

ATG，自噬相关；BioID，邻近依赖性生物素化；CM，条件培养基；EV，细胞外囊泡；HNRNPK，异质核核蛋白K；ILV，腔内囊泡；LDELS，依赖LC3的EV装载和分泌；LIR，LC3相互作用区；MAP1LC3 / LC3，微管相关蛋白1轻链3；MS，质谱； MS。MVB，多囊泡体；ncRNA，非编码RNA；NSMAF / FAN，中性鞘磷脂酶激活相关因子；P机构，加工机构；PE，磷脂酰乙醇胺；RB1CC1 / FIP200，RB1感应线圈1; RBP，RNA结合蛋白；RNA-seq，RNA测序；SAFB，支架连接因子B；SILAC，细胞培养物中氨基酸的稳定同位素标记；SMPD3 / nSMase2，鞘磷脂磷酸二酯酶3；TEM，透射电子显微镜；TMT，串联质量标记