Key message

The central cell withstands heat stress better than the egg and antipodal cells. Insilco analysis of transcriptomic data identified several heat responsive genes which are central cell specific.

Abstract

Crop damage due to heat stress (HS) is a major cause of yield lost. Plants are particularly susceptible to negative effects of HS during gametophyte development and fertilization. Extensive studies have been performed on the male gametophyte under HS, but how the female gametophyte copes with HS is largely unknown. To learn how the different cell types of the female gametophyte reacts to HS, we studied unfertilized CDC123::H2B:YFP ovules. We found that the YFP-specific florescent signal persisted in the central cell during HS significantly more than the egg cell. We also found that the fluorescent signal persistence was the lowest in the antipodal cells. This finding suggests that the reaction of the female gametophyte to HS is rather unique and differentially mediated according to the cell’s identity. In addition, mining through published transcriptomic datasets we found that several important heat stress responsive genes which are extremely upregulated during HS (more than 64-fold) are specifically expressed in the CC but not in the EC. Further research such as comparative transcriptomics and cell biology will likely shed more light on the phenomena reported here and increase our basic understandings about the ways sexual reproduction processes are affected by heat stress.

中文翻译：

---

拟南芥雌配子体在热应激期间观察到差异的细胞持久性。

关键信息

中央细胞比鸡蛋和对立细胞更好地承受热应激。转录组数据的硅胶分析鉴定了几个中央细胞特异性的热响应基因。

抽象

由于热胁迫（HS）造成的作物损害是产量损失的主要原因。植物在配子体发育和受精过程中特别容易受到HS的负面影响。在HS下对雄配子体进行了广泛的研究，但是雌配子体如何应对HS仍是未知的。为了了解雌性配子体的不同细胞类型对HS的反应，我们研究了未受精的CDC123 :: H2B：YFP胚珠。我们发现，在HS期间，YFP特异的荧光信号在中央细胞中持续存在的时间明显多于卵细胞。我们还发现，荧光信号的持久性在对映体细胞中最低。这一发现表明雌性配子体对HS的反应相当独特，并根据细胞的身份进行了不同程度的介导。此外，通过公开的转录组数据集挖掘，我们发现在CC中特异性上调了几个重要的热应激反应基因（大于64倍），但CC中却没有表达。诸如比较转录组学和细胞生物学之类的进一步研究可能会更多地揭示此处报道的现象，并增加我们对热应激影响性生殖过程的方式的基本认识。