An analytical method based on solid-phase extraction–hydrophilic interaction chromatography–tandem mass spectrometry (SPE–HILIC–MS/MS) has been developed to quantify moniliformin in vegetable oil. The sample was extracted by methanol and purified by an Oasis MAX solid-phase extraction column. Following purification, it was separated by a Waters HILIC hydrophilic interaction column. Acetonitrile and 0.5 mM ammonium acetate solution were used as a mobile phase for gradient elution. An electrospray-negative ion multiple-reaction monitoring mode was used to quantify moniliformin by external standard method. In the mass concentration range of 0.1–10 μg/L, the correlation coefficient of moniliformin was 0.9997. The detection limit of this method was 0.03 μg/L, and the limit of quantification was 0.1 μg/L. When the three levels of 1.0, 2.0 and 5.0 μg/kg moniliformin were added, the recovery was 84.6%–98.2% with a relative standard deviation of 1.3%–5.1%. The results indicated that this method is simple, fast, sensitive, reproducible, and can be used to determine moniliformin in vegetable oil.

中文翻译：

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固相萃取-亲水作用色谱-串联质谱法测定植物油中的念珠菌素

已开发出一种基于固相萃取-亲水相互作用色谱-串联质谱 (SPE-HILIC-MS/MS) 的分析方法来定量植物油中的念珠菌素。样品经甲醇萃取，经Oasis MAX固相萃取柱纯化。纯化后，通过 Waters HILIC 亲水相互作用柱进行分离。乙腈和 0.5 mM 醋酸铵溶液用作梯度洗脱的流动相。外标法采用电喷雾-负离子多反应监测模式对念珠菌素进行定量。在0.1-10 μg/L的质量浓度范围内，念珠菌素的相关系数为0.9997。该方法的检出限为0.03 μg/L，定量限为0.1 μg/L。当 1.0、2.0 和 5 三个级别时。添加0 μg/kg念珠菌素，回收率为84.6%~98.2%，相对标准偏差为1.3%~5.1%。结果表明，该方法简便、快速、灵敏、重现性好，可用于植物油中念珠菌素的测定。