This study aimed to express an inulinase gene from the yeast Kluyveromyces marxianus (KmINU) in Aurantiochytrium sp. and realized one-step utilization of inulin resource for DHA production without any chemical pretreatment. An expression cassette with a length of 6052 bp for expressing the inulinase gene was constructed by a fast two-step PCR method and then was transferred into the Aurantiochytrium sp. cells. The Aurantiochytrium sp. recombinant T39 was selected with an inulinase activity up to 50.1 U/mL in 72 h. In a 5-l fed-batch fermentation, as high as 148.9 g/L of inulin was directly used within 120 h, and only 1.2 g/L of total sugar was left in the medium at the end of fermentation. The biomass of 51.4 g/L with a lipid content of 69.2% DCW and a DHA yield of 14.9 g/L was obtained.

中文翻译：

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菊粉的一步利用，用于通过重组金丝壶菌属菌种生产二十二碳六烯酸（DHA）。携带马克斯克鲁维酵母菊粉酶。

这项研究旨在从Aurantiochytrium sp。中的酵母克鲁维酵母（KmYU）表达菊粉酶基因。无需任何化学预处理即可实现菊粉资源一步生产DHA的生产。通过快速的两步PCR方法构建了一个用于表达菊粉酶基因的表达盒，该表达盒的长度为6052 bp，然后将其转移到Aurantiochytrium sp中。细胞。Aurantiochytrium sp。在72小时内选择了菊粉酶活性高达50.1 U / mL的重组T39。在5升分批补料发酵中，在120小时内直接使用了高达148.9 g / L的菊粉，在发酵结束时培养基中只剩下1.2 g / L的总糖。获得了51.4 g / L的生物量，脂质含量为69.2％DCW，DHA产量为14.9 g / L。