Degradation studies of phenanthrene and naphthalene as a mixture was carried out using a developed bacterial consortium. The isolates used in consortium were identified as Chryseobacterium sp., Sphingobacterium sp., Stenotrophomonas sp., Agromyces sp. and Pseudomonas sp. Limited work is done on genus Agromyces in degradation studies of PAHs. Catechol production was detected using Arnow’s assay suggested that the pathway used for degradation is the meta-cleavage pathway. Results showed that Tween 80, as a surfactant, had maximum effect on the growth of isolates during PAH degradation. This suggests that use of Tween 80 as a surfactant enhanced the uptake of PAH by bacterial isolates during degradation. The study further revealed that, bacterial consortium was successfully utilized in the treatment of water contaminated with PAH in a laboratory-scale biofilm bioreactor. The bacterial consortium was able to degrade 99.9% of naphthalene and 92.9% of phenanthrene as a mixture at a high concentration of 2000 mg/L within 6 days. Further scaling up of the biofilm bioreactor can prove beneficial in large scale treatment of PAH contaminated water. This study showed promising results and these bacterial strains can be used as potential tools for bioremediation of PAH in contaminated sites.

使用发达的细菌联盟对菲和萘的混合物进行降解研究。在联盟中所使用的菌株被确定为金黄属，鞘氨醇菌属，嗜菌，Agromyces SP。和假单胞菌sp。在PAHs降解研究中，农杆菌属的研究工作很少。使用Arnow分析检测到邻苯二酚的产生，表明降解所用的途径是元裂解途径。结果表明，吐温80作为表面活性剂，对PAH降解过程中分离株的生长具有最大的影响。这表明使用吐温80作为表面活性剂可增强细菌分离株在降解过程中对PAH的吸收。研究进一步表明，在实验室规模的生物膜生物反应器中，细菌财团已成功用于处理被PAH污染的水。细菌财团能够在6天内以2000 mg / L的高浓度降解99.9％的萘和92.9％的菲混合物。生物膜生物反应器的进一步放大可以证明在大规模处理PAH污染水方面是有益的。这项研究显示出令人鼓舞的结果，这些细菌菌株可以用作在受污染场所进行PAH生物修复的潜在工具。