Abstract Although most lactic acid bacteria do not directly incorporate purine nucleotides, the strain Lactobacillus gasseri PA-3 was found to incorporate purine mononucleotides. To determine whether the direct uptake of purine mononucleotides is dependent on the species or strain of lactic acid bacteria, incorporation of purine mononucleotides was assessed in L. gasseri, Lactcoccus lactis sbsp. lactis, Streptococcus thermophilus and other species of lactic acid bacteria. Each bacterial strain was incubated with 32P-AMP or 14C-adenosine and the incorporation of each purine was evaluated by measuring their radioactivity. All investigated strains of L. gasseri incorporated 32P-AMP, whereas strains of S. thermophilus and most strains of L. lactis did not. Incorporation of 32P-AMP into strains of Pediococcus was dependent on the strain or species of that genus of bacteria. All investigated strains, except for one strain of L. gasseri, incorporated 14C-adenosine, with S. thermophilus, L. lactis and Pediococcus generally displaying greater incorporation of 14C-adenosine than L. gasseri. Although most lactic acid bacteria such as S. thermophiles and L. lactis do not incorporate purine mononucleotides, some species such as L. gasseri directly incorporate purine mononucleotides. These findings indicate that the preferential incorporation of purine mononucleotides or nucleosides by lactic acid bacteria is dependent on the species or strain.

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乳酸菌掺入嘌呤单核苷酸和核苷的物种依赖性模式

摘要 尽管大多数乳酸菌不直接掺入嘌呤核苷酸，但发现加氏乳杆菌 PA-3 菌株掺入了嘌呤单核苷酸。为了确定嘌呤单核苷酸的直接摄取是否取决于乳酸菌的种类或菌株，在 L.gasseri、Lactcoccus lactis sbsp 中评估了嘌呤单核苷酸的掺入。乳酸菌、嗜热链球菌和其他乳酸菌。将每种细菌菌株与 32P-AMP 或 14C-腺苷一起温育，并通过测量它们的放射性来评估每种嘌呤的掺入情况。所有研究的 L.gasseri 菌株都掺入了 32P-AMP，而嗜热链球菌菌株和大多数乳酸乳球菌菌株则没有。将 32P-AMP 掺入片球菌菌株取决于该细菌属的菌株或物种。除了加氏乳杆菌的一种菌株外，所有研究的菌株都掺入了 14C-腺苷，嗜热链球菌、乳酸乳球菌和片球菌通常显示出比加氏乳杆菌更多的 14C-腺苷掺入。尽管大多数乳酸菌如嗜热链球菌和乳酸乳球菌不掺入嘌呤单核苷酸，但一些物种如加氏乳杆菌直接掺入嘌呤单核苷酸。这些发现表明乳酸菌优先掺入嘌呤单核苷酸或核苷取决于物种或菌株。乳酸菌和片球菌通常比 L.gasseri 表现出更多的 14C-腺苷掺入。尽管大多数乳酸菌如嗜热链球菌和乳酸乳球菌不掺入嘌呤单核苷酸，但一些物种如加氏乳杆菌直接掺入嘌呤单核苷酸。这些发现表明乳酸菌优先掺入嘌呤单核苷酸或核苷取决于物种或菌株。乳酸菌和片球菌通常比 L.gasseri 表现出更多的 14C-腺苷掺入。尽管大多数乳酸菌如嗜热链球菌和乳酸乳球菌不掺入嘌呤单核苷酸，但一些物种如加氏乳杆菌直接掺入嘌呤单核苷酸。这些发现表明乳酸菌优先掺入嘌呤单核苷酸或核苷取决于物种或菌株。