In the present study, the composition of the extracellular matrix (ECM) of the biofilm formed by Scedosporium apiospermum, S. aurantiacum, S. minutisporum and Lomentospora prolificans on a polystyrene surface was investigated. Confocal laser scanning microscopy revealed a dense mycelial mass, with an ECM covering/interspersing the fungal cells and containing carbohydrate-rich molecules (e.g. glycoproteins) and extracellular DNA. The ECMs that were chemically extracted from mature biofilms formed by each of these fungi was predominantly composed of polysaccharides, followed by proteins, nucleic acids and sterols. In general, the amount of biofilm ECM was significantly greater in S. minutisporum and S. aurantiacum than in S. apiospermum and L. prolificans. Corroborating these results, the disarticulation of mature biofilms with enzymes, sodium metaperiodate and chelating agents occurred mainly in S. minutisporum and S. aurantiacum. Collectively, these results have revealed for the first time the composition of the ECM of the biofilms formed by Scedosporium/Lomentospora species and the role it plays in their architecture.

在本研究中，研究了在聚苯乙烯表面上由油菜假单孢菌（Sedosporium apiospermum），金黄色葡萄球菌（S. aurantiacum），小叶葡萄球菌（S. minutisporum）和多色Lomentospora prolificans形成的生物膜的细胞外基质（ECM）的组成。共聚焦激光扫描显微镜显示菌丝体密集，ECM覆盖/散布在真菌细胞中，并含有富含碳水化合物的分子（例如糖蛋白）和细胞外DNA。从每种真菌形成的成熟生物膜中化学提取的ECM主要由多糖组成，其次是蛋白质，核酸和固醇。总的来说，在S. minutisporum和S. aurantiacum中，生物膜ECM的数量明显增加。而不是在S. apiospermum和L. prolificans中。这些结果证实了成熟的生物膜与酶，偏高碘酸钠和螯合剂的脱节主要发生在S. minutisporum和S. aurantiacum中。总的来说，这些结果首次揭示了由景天孢子/门孢菌属物种形成的生物膜的ECM组成及其在结构中的作用。