Abstract The determination of vitamin E as chemical marker in Spirulina platensis hydroalcoholic extracts and creams is reported by the use of high-performance liquid chromatography (HPLC) and multi-syringe chromatography (MSC). The validation of the HPLC method using a methanol as the mobile phase showed linearity (r = 0.9994), a quantification limit of 1.25 µg mL−1, favorable precision with a relative standard deviation less than 1.5%, and a recovery of 98.9%. The samples were pretreated with potassium hydroxide/ethanol and extracted with petroleum ether. The resulting solid residue was dissolved in methanol before analysis. MSC was developed using a monolithic column coupled to a multi-syringe system. Both on-line and pretreated samples were analyzed by this method. The results obtained for the hydroalcoholic extract of S. platensis by HPLC and MSC were in agreement.

中文翻译：

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采用多注射器色谱 (MSC) 和高效液相色谱 (HPLC) 测定钝顶螺旋藻提取物和光保护霜中的维生素 E

摘要 本文报道了使用高效液相色谱 (HPLC) 和多注射器色谱 (MSC) 测定螺旋藻水醇提取物和乳膏中作为化学标志物的维生素 E。使用甲醇作为流动相的 HPLC 方法验证显示线性 (r = 0.9994)，定量限为 1.25 µg mL-1，精密度良好，相对标准偏差小于 1.5%，回收率为 98.9%。样品用氢氧化钾/乙醇预处理并用石油醚萃取。在分析前将所得固体残余物溶解在甲醇中。MSC 是使用与多注射器系统耦合的整体柱开发的。在线样品和预处理样品均通过该方法进行分析。S. 水醇提取物的结果。