Florida torreya (Torreya taxifolia Arn.) is an endangered conifer with a very limited range in the USA: two counties in Florida and one in Georgia, along the Apalachicola River. The species was once abundant in its small native range but suffered a major decline, ~99% loss, in the late 1950s to early 1960s that is thought to have been caused by a disease. Recently, a canker disease caused by Fusarium torreyae was identified as the primary cause of Florida torreya decline. Efforts to restore and preserve the species in situ and ex situ are hampered by lack of pathogen‐free planting stock, and there exists an interest in methods to verify pathogen presence in seeds and seedlings prior to collection and transport for planting. This paper presents a new species‐specific diagnostic method that enables detection of F. torreyae and may allow for conservation programmes to ensure germplasm is free of the pathogen prior to planting.

中文翻译：

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佛罗里达州榧、Torreya taxfolia 溃疡病菌Fusarium torreyae 的检测方法

佛罗里达榧树 (Torreya taxfolia Arn.) 是一种濒临灭绝的针叶树，在美国的分布范围非常有限：佛罗里达州的两个县和乔治亚州的一个县，位于阿巴拉契科拉河沿岸。该物种曾经在其小型原生范围内丰富，但在 1950 年代末至 1960 年代初遭受了重大下降，约 99% 的损失，这被认为是由疾病引起的。最近，由榧木镰刀菌引起的溃疡病被确定为佛罗里达州榧树衰退的主要原因。由于缺乏无病原体的种植种群，在原地和异地恢复和保护物种的努力受到阻碍，人们对在收集和运输种植之前验证种子和幼苗中病原体存在的方法感兴趣。本文提出了一种新的物种特异性诊断方法，可以检测 F.