This study aimed to investigate the effects of heavy metals, such as copper, zinc, iron, cobalt, chromium, aluminium, manganese and molybdenum, on zebrafish egg hatching rate and the subsequent survival rate of the larvae. Each metal was used in the various concentrations of0.05 mg/L, 50 mg/L and 500 mg/L to detect the threshold limit of tolerance. The study's results showed significant differences in the hatching and survival rate of larvae between the control group and most of the experimental groups when adding heavy metals in various concentrations. The highest concentration (500 mg/L) of the induced heavy metals tested in this study resulted in total egg or larval mortality within 96 hr and significantly lower egg or larval life expectancy. Among the tested metals, zinc and molybdenum had the most significant impact on hatching time, in contrast to iron that did not differentiate the results from the control group. Copper, cobalt, chromium, aluminium and manganese did affect zebrafish eggs depending on their exposure concentrations, which differentiated the results from the control group. Also, the study's findings demonstrate a significant dependence of the hatched egg heart rate on the metal concentration, and in particular, higher concentrations of metals correspond to higher heart rate. Zinc, Molybdenum and copper was proved to be the most toxic heavy metals causing increased hazard rate to mortality up to 96 hr and shorter life expectancy. Finally, skeletal deformities that occasionally observed did not significantly affect the total life expectancy of the hatched eggs.

中文翻译：

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重金属对斑马鱼胚胎发育影响的比较研究

这项研究旨在研究重金属，如铜，锌，铁，钴，铬，铝，锰和钼，对斑马鱼卵孵化率和幼虫随后存活率的影响。每种金属分别以0.05 mg / L，50 mg / L和500 mg / L的浓度使用，以检测耐受性的阈限。研究结果表明，当添加不同浓度的重金属时，对照组和大多数实验组的幼虫孵化率和存活率存在显着差异。在这项研究中测试的最高浓度（500 mg / L）的诱导重金属导致卵或幼虫的总死亡率在96小时内，并且显着降低卵或幼虫的预期寿命。在测试的金属中，锌和钼对孵化时间的影响最大，与铁相比，铁与对照组没有区别。铜，钴，铬，铝和锰确实会影响斑马鱼卵，具体取决于它们的暴露浓度，这与对照组的结果有所不同。此外，该研究结果还表明，孵化的卵心率对金属浓度有显着依赖性，特别是较高的金属浓度对应于较高的心率。事实证明，锌，钼和铜是最有毒的重金属，可导致高达96小时的死亡率上升和较短的预期寿命。最后，偶尔观察到的骨骼畸形不会显着影响孵化卵的总寿命。铬，铝和锰确实会影响斑马鱼卵，具体取决于它们的暴露浓度，这与对照组的结果有所不同。此外，该研究结果还表明，孵化的卵心率对金属浓度有显着依赖性，特别是较高的金属浓度对应于较高的心率。事实证明，锌，钼和铜是最有毒的重金属，可导致高达96小时的死亡率上升和较短的预期寿命。最后，偶尔观察到的骨骼畸形不会显着影响孵化卵的总寿命。铬，铝和锰确实会影响斑马鱼的卵，具体取决于它们的暴露浓度，这与对照组的结果有所不同。此外，该研究结果还表明，孵化的卵心率对金属浓度有显着依赖性，特别是较高的金属浓度对应于较高的心率。事实证明，锌，钼和铜是最有毒的重金属，可导致高达96小时的死亡率上升和较短的预期寿命。最后，偶尔观察到的骨骼畸形不会显着影响孵化卵的总寿命。研究结果表明，孵化的鸡蛋心率对金属浓度的依赖性很大，特别是较高的金属浓度对应较高的心率。事实证明，锌，钼和铜是最有毒的重金属，可导致高达96小时的死亡率上升和较短的预期寿命。最后，偶尔观察到的骨骼畸形不会显着影响孵化卵的总寿命。研究结果表明，孵化后的鸡蛋心率对金属浓度的依赖性很大，特别是较高的金属浓度对应较高的心率。事实证明，锌，钼和铜是最有毒的重金属，可导致高达96小时的死亡率上升和较短的预期寿命。最后，偶尔观察到的骨骼畸形不会显着影响孵化卵的总寿命。