Infection with barley yellow dwarf virus (BYDV), caused by strains of virus belonging to the family Luteovirideae including BYDV‐PAV, can result in significant yield losses in autumn sown cereals following transmission by aphid vectors such as Rhopalosiphum padi (Homoptera: Aphididae) and Sitobion avenae (Homoptera: Aphididae). Spatial and temporal variance in the infectivity of alate populations influences risk to crops from the disease, which is greatest on infection at early crop growth stages. A decision support system (DSS) to guide optimised integration of crop protection strategies through risk assessment would help avoid unnecessary application of synthetic insecticides. This study contributes to the development of a DSS by exploring the viability and relevance of a methodology to detect virus levels in individual aphids trapped in‐crop using yellow sticky traps. Using a reverse transcription polymerase chain reaction (RT‐PCR) assay, the detectability of virus from a BYDV‐PAV‐positive control colony was found not to be reduced by the process of trapping, extraction and cold storage, but did drop significantly after between three and seven days of exposure on trap. This method has potential to contribute to localised risk assessment and guide optimisation of crop protection strategies.

中文翻译：

---

对种植在黄色粘性陷阱上的蚜虫进行 PCR 检测能否为田间大麦黄矮病毒 (BYDV) 风险评估提供信息？

大麦黄矮病毒 (BYDV) 由属于包括 BYDV-PAV 在内的黄体病毒科病毒株引起的感染可导致秋播谷物在通过蚜虫载体（如 Rhopalosiphum padi（同翅目：蚜科）和Sitobion avenae（同翅目：蚜科）。Alate 种群传染性的时空变异会影响作物受到该病害的风险，这在作物早期生长阶段的感染中最为明显。决策支持系统 (DSS) 通过风险评估指导作物保护策略的优化整合，将有助于避免不必要地使用合成杀虫剂。本研究通过探索使用黄色粘性陷阱检测作物中捕获的单个蚜虫中病毒水平的方法的可行性和相关性，为 DSS 的开发做出了贡献。使用逆转录聚合酶链反应 (RT-PCR) 分析，发现 BYDV-PAV 阳性对照菌落的病毒检测率并未因捕获、提取和冷藏过程而降低，但在在陷阱上暴露了三天和七天。这种方法有可能有助于局部风险评估并指导作物保护策略的优化。提取和冷藏，但在陷阱暴露 3 到 7 天后确实显着下降。这种方法有可能有助于局部风险评估并指导作物保护策略的优化。提取和冷藏，但在陷阱暴露 3 到 7 天后确实显着下降。这种方法有可能有助于局部风险评估并指导作物保护策略的优化。