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MiR-216a-3p suppresses the proliferation and invasion of cervical cancer through downregulation of ACTL6A-mediated YAP signaling.
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-05-13 , DOI: 10.1002/jcp.29783 Juan Zhao 1 , Long Li 1 , Ting Yang 1
Journal of Cellular Physiology ( IF 4.5 ) Pub Date : 2020-05-13 , DOI: 10.1002/jcp.29783 Juan Zhao 1 , Long Li 1 , Ting Yang 1
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The tumor‐suppressive role of microRNA‐216a‐3p (miR‐216a‐3p) has been evidenced in multiple tumors. Yet, the relevance of miR‐216a‐3p in cervical cancer remains undermined. The current study was designed to determine the expression and potential function of miR‐216a‐3p in cervical cancer. Expression of miR‐216a‐3p was markedly decreased in cervical cancer and functional assays revealed an inhibitory effect of miR‐216a‐3p on the proliferation, colony formation, and invasion of cervical cancer. Actin‐like 6A (ACTL6A) was identified as a target gene of miR‐216a‐3p. Elevated ACTL6A expression was detected in cervical cancer, and ACTL6A inhibition exhibited a tumor‐suppressive effect. ACTL6A inhibition increased yes‐associated protein (YAP) phosphorylation and downregulated YAP‐mediated transcriptional activity. ACTL6A restoration or YAP reactivation partially abrogated the miR‐216a‐3p‐mediated antitumor effect in cervical cancer cells. Taken together, these data demonstrate that miR‐216a‐3p acts as a potential tumor‐suppressive miRNA in cervical cancer, which exerts its function through inhibition of YAP signaling via targeting ACTL6A.
中文翻译：
MiR-216a-3p通过下调ACTL6A介导的YAP信号传导抑制宫颈癌的增殖和侵袭。
microRNA‐216a‐3p（miR‐216a‐3p）的肿瘤抑制作用已在多种肿瘤中得到证实。然而，miR‐216a‐3p在宫颈癌中的相关性仍然被削弱。本研究旨在确定miR‐216a‐3p在宫颈癌中的表达和潜在功能。在宫颈癌中，miR‐216a‐3p的表达明显降低，功能测定显示miR‐216a‐3p对宫颈癌的增殖，集落形成和侵袭具有抑制作用。肌动蛋白样6A（ACTL6A）被鉴定为miR‐216a‐3p的靶基因。在宫颈癌中检测到ACTL6A表达升高，并且ACTL6A抑制作用表现出抑癌作用。ACTL6A抑制作用增加了是相关蛋白（YAP）的磷酸化，并下调了YAP介导的转录活性。ACTL6A的恢复或YAP的激活在一定程度上废除了miR‐216a‐3p介导的对宫颈癌细胞的抗肿瘤作用。综上所述，这些数据表明miR‐216a‐3p可作为宫颈癌中潜在的抑癌miRNA，通过靶向ACTL6A抑制YAP信号传导发挥其功能。
更新日期：2020-05-13
中文翻译：
MiR-216a-3p通过下调ACTL6A介导的YAP信号传导抑制宫颈癌的增殖和侵袭。
microRNA‐216a‐3p（miR‐216a‐3p）的肿瘤抑制作用已在多种肿瘤中得到证实。然而，miR‐216a‐3p在宫颈癌中的相关性仍然被削弱。本研究旨在确定miR‐216a‐3p在宫颈癌中的表达和潜在功能。在宫颈癌中，miR‐216a‐3p的表达明显降低，功能测定显示miR‐216a‐3p对宫颈癌的增殖，集落形成和侵袭具有抑制作用。肌动蛋白样6A（ACTL6A）被鉴定为miR‐216a‐3p的靶基因。在宫颈癌中检测到ACTL6A表达升高，并且ACTL6A抑制作用表现出抑癌作用。ACTL6A抑制作用增加了是相关蛋白（YAP）的磷酸化，并下调了YAP介导的转录活性。ACTL6A的恢复或YAP的激活在一定程度上废除了miR‐216a‐3p介导的对宫颈癌细胞的抗肿瘤作用。综上所述，这些数据表明miR‐216a‐3p可作为宫颈癌中潜在的抑癌miRNA，通过靶向ACTL6A抑制YAP信号传导发挥其功能。
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