Human papillomavirus (HPV) is the most common sexually transmitted viral infection worldwide. HPV tumorigenesis genotypes are the causative agents of cervical cancer and genital malignancies. The scientific literature has demonstrated that life style, environmental, epigenetic accompanied with HR‐HPV genotypes are potential risk factors for cervical cancer progression. The frequencies of the Cyp2C9*², Cyp2C9*³, and vitamin K epoxide reductase complex subunit 1 (VKORC1) genotypes as potential molecular biomarkers have been investigated on Iranian women with cervical malignancy related to HPV genotypes. As a case‐control study, the mutations were appraised using a polymerase chain reaction‐restriction fragment length polymorphism procedure on women suffering from HPV infection (60 cases), CC (46 cases), and 40 subjects of as healthy control. The outcomes demonstrated that Cyp2C9*³ showed a meaningful relationship between women diagnosed with cervical cancer and the healthy population (AA vs. AC; OR, 7.15; 95% CI, 1.94‐26.3; p = .003). It was also observed that the Cyp2C9*³ mutation in women with cervical cancer and VKORC1 in healthy population with HPV (+), did not follow the Hardy–Weinberg equilibrium. Our findings aid understanding the genetic polymorphism distribution of Cyp2C9*², Cyp2C9*³, and VKORC1 in women with genital malignancies. This can also be useful in predicting the susceptibility risk factors for developing cervical cancer. However, allelic discrimination as a molecular biomarker requires further research.

中文翻译：

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HPV 相关宫颈癌女性 Cyp2C9*2 (Arg144Cys C>T)、Cyp2C9*3 (Ile359Leu A>C) 和 VKORC1 (1639G>A) 点突变分析：病例对照研究。


人乳头瘤病毒（HPV）是全世界最常见的性传播病毒感染。 HPV 肿瘤发生基因型是宫颈癌和生殖器恶性肿瘤的致病因子。科学文献表明，生活方式、环境、表观遗传以及HR-HPV基因型是宫颈癌进展的潜在危险因素。 Cyp2C9* ² 、 Cyp2C9* ³和维生素 K 环氧还原酶复合物亚基 1 (VKORC1) 基因型作为潜在分子生物标志物的频率已在患有与 HPV 基因型相关的宫颈恶性肿瘤的伊朗妇女中进行了研究。作为一项病例对照研究，使用聚合酶链反应限制性片段长度多态性程序对患有 HPV 感染的女性（60 例）、CC（46 例）和 40 名健康对照受试者进行突变评估。结果表明，Cyp2C9* ³在诊断为宫颈癌的女性与健康人群之间显示出有意义的关系（AA 与 AC；OR，7.15；95% CI，1.94‐26.3； p = 0.003）。还观察到，宫颈癌女性中的 Cyp2C9* ³突变和 HPV (+) 健康人群中的 VKORC1 突变不遵循 Hardy-Weinberg 平衡。我们的研究结果有助于了解 Cyp2C9* ² 、 Cyp2C9* ³和 VKORC1 在患有生殖器恶性肿瘤的女性中的遗传多态性分布。这也可用于预测患宫颈癌的易感性危险因素。然而，等位基因区分作为分子生物标志物需要进一步研究。