Retinal photoreceptor degeneration occurs frequently in several neurodegenerative retinal diseases such as age-related macular degeneration, retinitis pigmentosa, or genetic retinal diseases related to the photoreceptors. Despite the impact on daily life and the social and economic consequences, there is no cure for these diseases. Considering this, cell-based therapy may be an optimal therapeutic option. This study evaluated the neuroprotective in vitro potential of a secretome of human bone marrow mesenchymal stem cells (MSC) for retinal photoreceptors in vitro. We analyzed the photoreceptor morphologic changes and the paracrine factors secreted by human bone marrow MSC in a physically-separated co-culture with degenerated neuroretinas, using organotypic neuroretinal cultures. The results showed that the secretome of human bone marrow MSC had a neuroprotective effect over the neuroretinal general organization and neuropreserved the photoreceptors from degeneration probably by secretion of neuroprotective proteins. The study of the expression of 1000 proteins showed increased paracrine factors secreted by MSC that could be crucial in the neuroprotective effect of the stem cells secretome over in vitro retinal degeneration. The current results reinforce the hypothesis that the paracrine effect of the human bone marrow MSC may slow photoreceptor neurodegeneration and be a therapeutic option in retinal photoreceptor degenerative diseases.

视网膜光感受器变性经常发生在几种神经退行性视网膜疾病中，例如年龄相关性黄斑变性，色素性视网膜炎或与光感受器有关的遗传性视网膜疾病。尽管对日常生活产生了影响，并带来了社会和经济后果，但仍无法治愈这些疾病。考虑到这一点，基于细胞的疗法可能是最佳的治疗选择。本研究评估神经保护在体外人骨髓的分泌蛋白质组间充质干对于视网膜光感受器细胞（MSC）的电势在体外。我们使用器官型神经视网膜培养物，在与退化的神经视网膜进行物理分离的共培养物中，分析了人类骨髓MSC分泌的感光细胞形态变化和旁分泌因子。结果表明，人骨髓间充质干细胞的分泌组对神经视网膜的一般组织具有神经保护作用，并可能通过分泌神经保护蛋白而使感光细胞免于变性。对1000种蛋白质表达的研究表明，MSC分泌的旁分泌因子增加，这可能是干细胞分泌基因组对体外视网膜变性的神经保护作用的关键。