Many plant metabolism processes are currently not completely understood despite the numerous studies. These include the events in plant shoots and especially in the apical meristem. To understand the various mechanisms on a molecular level, a combined approach of target and non-targeted fingerprinting analysis was worked out on different white asparagus spear segments using high resolution mass spectrometry. By means of various multivariate analysis strategies, numerous distinctions within diverse substance classes were observed. While most of the investigated metabolites were present in relatively higher concentrations in the tip of the asparagus spears, others were more accumulated at the bottom, some, in turn, did not show any concentration differences along the shoot. Using pathway analysis, the most significant metabolites were classified in the biological context. To our knowledge for the first time, a non-targeted metabolomics approach is used with the aim of metabolic profiling of plant sprouts.

中文翻译：

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代谢成像：使用高分辨率质谱法分析白芦笋茎的不同部分

尽管进行了大量研究，但目前尚未完全了解许多植物代谢过程。这些包括植物芽中的事件，尤其是顶端分生组织中的事件。为了在分子水平上了解各种机制，使用高分辨率质谱法对不同的白芦笋矛段进行了目标和非目标指纹分析的组合方法。通过各种多变量分析策略，观察到不同物质类别中的许多区别。虽然大多数研究的代谢物在芦笋尖部以相对较高的浓度存在，但其他代谢物在底部积累更多，而有些则没有显示出沿枝条的任何浓度差异。使用通路分析，最重要的代谢物是在生物学背景下分类的。据我们所知，首次使用非靶向代谢组学方法对植物芽进行代谢分析。