Lamin B2 promotes the malignant phenotype of non-small cell lung cancer cells by upregulating dimethylation of histone 3 lysine 9.,Experimental Cell Research

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Lamin B2 promotes the malignant phenotype of non-small cell lung cancer cells by upregulating dimethylation of histone 3 lysine 9.
Experimental Cell Research ( IF 3.3 ) Pub Date : 2020-05-13 , DOI: 10.1016/j.yexcr.2020.112090
Mei-Yu Zhang ₁ , Yu-Chen Han ₂ , Qiang Han ₃ , Yuan Liang ₁ , Yuan Luo ₁ , Lai Wei ₁ , Ting Yan ₁ , Yue Yang ₁ , Shu-Li Liu ₃ , En-Hua Wang ₃

Affiliation

The relationship between Lamin B2 and tumor proliferation and migration is unclear. We explored the impact of Lamin B2 on non-small cell lung cancer (NSCLC) cells. Tissue microarray and immunohistochemistry were combined to evaluate Lamin B2 expression and its relationship with the clinicopathological factors found in NSCLC. Western blotting, immunofluorescence analysis, and bioinformatics were used to investigate the effects of Lamin B2 on various regulatory pathways in cancer. Cytological experiments were conducted to evaluate Lamin B2 expression in tumor cells. We conducted co-immunoprecipitation and chromatin immunoprecipitation to explore the molecular mechanisms underlying the relationship between Lamin B2 and NSCLC and evaluate the results of rescue experiments. Lamin B2 was highly expressed in NSCLC and positively correlated with lymph node metastasis. In NSCLC, Lamin B2 interacted with Cyclin D1, upregulating G9α expression, thus increasing H3K9me2 levels. H3K9me2 binds to the promoter region of the E-cadherin gene (CDH1) to induce CDH1 silencing and promotes cancer cell migration. Thus, we found that Lamin B2 was highly expressed in NSCLC cells and promoted their migration by increasing H3K9me2 levels, which induced E-cadherin gene silencing.

中文翻译：

Lamin B2 通过上调组蛋白 3 赖氨酸 9 的二甲基化促进非小细胞肺癌细胞的恶性表型。

Lamin B2 与肿瘤增殖和迁移之间的关系尚不清楚。我们探讨了 Lamin B2 对非小细胞肺癌 (NSCLC) 细胞的影响。组织微阵列和免疫组织化学结合评估 Lamin B2 表达及其与 NSCLC 中发现的临床病理因素的关系。使用蛋白质印迹、免疫荧光分析和生物信息学研究 Lamin B2 对癌症各种调节途径的影响。进行细胞学实验以评估肿瘤细胞中核纤层蛋白B2的表达。我们进行了免疫共沉淀和染色质免疫沉淀，以探讨 Lamin B2 与 NSCLC 之间关系的分子机制，并评估挽救实验的结果。 Lamin B2在NSCLC中高表达，且与淋巴结转移呈正相关。在 NSCLC 中，Lamin B2 与 Cyclin D1 相互作用，上调 G9α 表达，从而增加 H3K9me2 水平。 H3K9me2 与 E-钙粘蛋白基因 (CDH1) 的启动子区域结合，诱导 CDH1 沉默并促进癌细胞迁移。因此，我们发现Lamin B2在NSCLC细胞中高表达，并通过增加H3K9me2水平促进其迁移，从而诱导E-钙粘蛋白基因沉默。

更新日期：2020-05-13

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