A new type of ultrathin fiber microprobe for selective electroporation is reported. The microprobe is 10 cm long and has a diameter of 350 µm. This microprobe is a low cost tool, which allows electroporation of an arbitrary selected single cell or groups of cells among population with use of a standard microscope and cell culture plates. The microprobe in its basic form contains two metal microelectrodes made of a silver-copper alloy, running along the fiber, each with a diameter of 23 µm. The probe was tested in vitro on a population of normal and cancer cells. Successful targeted electroporation was observed by means of accumulation of trypan blue (TB) dye marker in the cell. The electroporation phenomenon was also verified with propidium iodide and AnnexinV in fluorescent microscopy.

中文翻译：

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超薄玻璃纤维微探针，用于电穿孔任意选定的细胞组。

报道了一种用于选择性电穿孔的新型超细纤维微探针。微型探针长10厘米，直径350 µm。该微探针是一种低成本的工具，它允许使用标准显微镜和细胞培养板对人群中任意选择的单个细胞或细胞组进行电穿孔。基本形状的微探针包含两个由银铜合金制成的金属微电极，沿着金属纤维分布，每个电极的直径为23 µm。该探针在正常细胞和癌细胞群体上进行了体外测试。通过台盼蓝（TB）染料标记在细胞中的积累，观察到成功的靶向电穿孔。还用碘化丙锭和AnnexinV在荧光显微镜下验证了电穿孔现象。