Integral membrane proteins (IMPs) perform unique and indispensable functions in the cell, making them attractive targets for fundamental research and drug discovery. Developments in protein production, isotope labeling, sample preparation, and pulse sequences have extended the utility of solution NMR spectroscopy for studying IMPs with multiple transmembrane segments. Here we review some recent applications of solution NMR for studying structure, dynamics, and interactions of polytopic IMPs, emphasizing strategies used to overcome common technical challenges.

中文翻译：

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膜蛋白的溶液NMR光谱。

整合膜蛋白（IMP）在细胞中执行独特且必不可少的功能，使其成为基础研究和药物发现的有吸引力的靶标。蛋白质生产，同位素标记，样品制备和脉冲序列的发展已经扩展了溶液NMR光谱学在研究具有多个跨膜片段的IMPs方面的实用性。在这里，我们回顾了溶液NMR在研究多分子IMP的结构，动力学和相互作用方面的最新应用，重点介绍了用于克服常见技术挑战的策略。