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Identification of Fusarium wilt resistance sources in melon (Cucumis melo L.) landraces of Iran using marker-assisted selection technique
Australasian Plant Pathology ( IF 0.9 ) Pub Date : 2020-05-12 , DOI: 10.1007/s13313-020-00713-9
Ahmad Mahdavi Meighan , Babak Rabiei , Seyyed Akbar Khodaparast

Melon (Cucumis melo L.) is an important popular high-consumption product in the world. Melon Fusarium wilt (Fusarium oxysporum f.sp. melonis: FOM) is a global destructive disease. In this research, 88 melon landraces from different geographical regions of Iran together with reference genotypes as control genotypes were screened for resistance to 0 and 1 races of Fusarium wilt disease agent using artificial inoculation and two functional markers for the presence or absence of Fom-2 gene. The landraces in multiple replicates were examined in a greenhouse experiment. Thirty-five out of the 88 landraces were selected to determine the disease incidence percentage (DIP), disease severity percentage (DSP) and area under disease progress curve (AUDPC). Based on the results of marker-assisted selection, 83 genotypes had at least one susceptible allele, while 43 heterozygote genotypes had at least one resistance allele. Among genotypes evaluated in artificial inoculation test, three landraces including Shahd-e-Shiraz Gosht Narenji, Korki and Alam Gorgor indicated the highest level of resistance. It seems that these landraces can be considered as the most useful sources of resistance in breeding programs for introducing Fusarium resistant melons. In contrast, Jalali, Petra falat and Habib-Abad landraces were the most sensitive genotypes, which can be used to produce sensitive standard check lines.

中文翻译:

使用标记辅助选择技术鉴定伊朗甜瓜 (Cucumis melo L.) 地方品种中的枯萎病抗性来源

甜瓜(Cucumis melo L.)是世界上重要的流行高消费产品。瓜类枯萎病(Fusarium oxysporum f.sp. melonis:FOM)是一种全球性破坏性疾病。在这项研究中,来自伊朗不同地理区域的 88 个甜瓜地方品种以及作为对照基因型的参考基因型使用人工接种和两种功能标记物筛选了对 0 和 1 种枯萎病病原体的抗性,以检测 Fom-2 的存在与否。基因。在温室实验中检查了多次重复的地方品种。从 88 个地方品种中选择了 35 个,以确定疾病发生率 (DIP)、疾病严重性百分比 (DSP) 和疾病进展曲线下面积 (AUDPC)。基于标记辅助选择的结果,83个基因型至少有一个易感等位基因,而43个杂合子基因型至少有一个抗性等位基因。在人工接种试验评估的基因型中,Shahd-e-Shiraz Gosht Narenji、Korki 和 Alam Gorgor 等三个地方品种表明抗性水平最高。似乎这些地方品种可以被认为是引入抗镰刀菌瓜类的育种计划中最有用的抗性来源。相比之下,Jalali、Petra falat 和 Habib-Abad 地方品种是最敏感的基因型,可用于产生敏感的标准检查系。似乎这些地方品种可以被认为是引入抗镰刀菌瓜类的育种计划中最有用的抗性来源。相比之下,Jalali、Petra falat 和 Habib-Abad 地方品种是最敏感的基因型,可用于产生敏感的标准检查系。似乎这些地方品种可以被认为是引入抗镰刀菌瓜类的育种计划中最有用的抗性来源。相比之下,Jalali、Petra falat 和 Habib-Abad 地方品种是最敏感的基因型,可用于产生敏感的标准检查系。
更新日期:2020-05-12
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