Due to the health benefits such as anti-diabetic, antioxidant, and anti-inflammatory agents, the extraction of bioactive compounds from natural plants becomes significant. One of the favored extraction processes is supercritical carbon dioxide (SC-CO₂). SC-CO₂ is known as a green method with a high-selectivity process. Therefore, this study aims to determine the best parameters and mass transfer process to extract the bioactive compounds, viz. sinensetin, isosinensetin, and rosmarinic acid, in the leaves of Orthosiphon stamineus using the SC-CO₂ extraction technique. Response surface methodology was applied to optimize the operating temperature and pressure of 40–80 °C and 10–30 MPa, respectively. Meanwhile, to quantify the concentration of sinensetin, isosinensetin, and rosmarinic acid, a high-performance liquid chromatography was used. Results showed that the level of sinensetin, isosinensetin, and rosmarinic acid varied significantly at each extraction condition from 122.98 to 469.54 mg/kg sample, 50.60 to 419.07 mg/kg sample, and 17.43 to 931.44 mg/kg, respectively. Based on the optimization study, the optimum pressure and temperature were 10 MPa and 80 °C, respectively, with 440.3 mg/g sinensetin, 392.9 mg/g isosinensetin, and 752.0 mg/g of rosmarinic acid concentration obtained. Meanwhile, the single-sphere model showed a significant correlation with sinensetin, isosinensetin, and rosmarinic acid extraction, with an average AARD of 12.31%, 13.72%, and 18.90%, respectively. The range of diffusivity coefficient, D_e, of sinensetin, isosinensetin, and rosmarinic acid was 5.47E−13–1.81E−12 m²/s, 8.26E−13–1.89E−12 m²/s, and 2.64E−13–2.28E−12 m²/s, respectively.

由于抗糖尿病，抗氧化剂和抗炎剂等健康益处，从天然植物中提取生物活性化合物变得意义重大。优选的萃取方法之一是超临界二氧化碳（SC-CO ₂）。SC-CO ₂被称为具有高选择性过程的绿色方法。因此，本研究旨在确定提取生物活性化合物的最佳参数和传质过程。sinensetin，isosinensetin和迷迭香酸，在叶肾茶使用SC-CO ₂提取技术。应用响应面方法分别优化了40–80°C和10–30 MPa的工作温度和压力。同时，为了定量测定芥子苷，异芥子苷和迷迭香酸的浓度，使用了高效液相色谱法。结果显示，在每种提取条件下，芥子素，异麦香素和迷迭香酸的水平在122.98至469.54 mg / kg样品，50.60至419.07 mg / kg样品和17.43至931.44 mg / kg的样品中变化显着。根据优化研究，获得的最佳压力和温度分别为10 MPa和80°C，所获得的芥子素浓度为440.3 mg / g，异芥子苷为392.9 mg / g，迷迭香酸浓度为752.0 mg / g。同时，单球模型显示出与sinensetin，isosinensetin，和迷迭香酸提取，平均AARD分别为12.31％，13.72％和18.90％。扩散系数范围芥末素，异芥子苷和迷迭香酸的D _e为5.47E-13–1.81E-12m ² /s、8.26E-13–1.89E-12m ² / s和2.64E-13–2.28E-12 m ² / s。