Apigenin is a traditional Chinese medicine found in many plants that plays critical roles in several diseases, including cardiovascular diseases. We investigated the protective effect of apigenin against ischemia/hypoxia (I/H)-induced myocardium injury in vitro and explored the potential molecular mechanisms. Together with the flow cytometry results, our results indicated that apigenin attenuated the pyroptosis and apoptosis of myoblastic H9c2 cells that were induced by I/H injury. Furthermore, the pro-inflammatory cytokines (interleukin 18, IL-18, and IL-1β) were investigated for the roles of apigenin in I/H-induced myocardium injury. It was observed that increases in IL-1β and IL-18 levels were significantly reduced by apigenin treatment of I/H-induced H9C2 cells. The results demonstrated that apigenin protected H9c2 cells against I/H-induced myocardium injury. The protective effects were most likely related to the reduction of pyroptosis, apoptosis, and pro-inflammatory cytokines.

中文翻译：

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芹菜素可通过介导细胞凋亡和凋亡来预防缺血/缺氧所致的心肌损伤。

芹菜素是在许多植物中发现的传统中药，在包括心血管疾病在内的多种疾病中起着至关重要的作用。我们调查芹菜素在体外对缺血/缺氧（I / H）引起的心肌损伤的保护作用，并探讨了潜在的分子机制。连同流式细胞仪结果，我们的结果表明芹菜素减轻了由I / H损伤诱导的成肌H9c2细胞的凋亡和凋亡。此外，研究了促炎细胞因子（白介素18，IL-18和IL-1β）在芹菜素在I / H诱导的心肌损伤中的作用。观察到芹菜素处理I / H诱导的H9C2细胞可显着降低IL-1β和IL-18水平的升高。结果表明芹菜素保护H9c2细胞免受I / H诱导的心肌损伤。保护作用最有可能与减少细胞凋亡，凋亡和促炎性细胞因子有关。