The mitochondrion is the target organelle for QoI (quinone outside inhibitor) antifungal drugs, but mitochondrial behavior in Pyricularia oryzae remains unclear. Conidial mitochondria in fungal isolate transformed with citrate synthase (CitA)-GFP fusion gene, were observed in situ using laser-scanning confocal microscopy and computer analysis. After mycelium was harvested at 36 h and aerial mycelia removed, conidia with 1, 2, or 3 cells were observed over time. The 3D visualization of mitochondria in these conidia showed that mitochondria were initially shaped like dots and developed into a network as the conidia aged.

中文翻译：

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使用绿色荧光蛋白（GFP）与柠檬酸合酶（CitA）融合的稻瘟病菌分生孢子线粒体的三维可视化

线粒体是QoI（醌外抑制剂）抗真菌药的靶细胞器，但米曲霉的线粒体行为尚不清楚。使用激光扫描共聚焦显微镜和计算机分析原位观察到了柠檬酸合酶（CitA）-GFP融合基因转化的真菌分离物中的分生孢子线粒体。在36小时收获菌丝体并除去气生菌丝体后，随时间观察到具有1、2或3个细胞的分生孢子。这些分生孢子中线粒体的3D可视化显示，线粒体最初呈点状，并随着分生孢子的老化而发展成网络。