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Optimisation of double-enzymatic extraction of arabinoxylan from fresh corn fibre
Journal of Food Science and Technology ( IF 3.1 ) Pub Date : 2020-05-13 , DOI: 10.1007/s13197-020-04502-6
Fumin Ma 1 , Xiaolei Li 1 , Jiayu Yin 2 , Lin Ma 1 , Dan Li 1
Affiliation  

Enzymatic extraction of arabinoxylans (AXs) is an attractive and environmentally friendly extraction option, in which technical considerations (yield and purity) have been coupled with environmental concerns. Amano HC 90 and Cellulase were combined to evaluate their interactive effects on AX extraction from destarched, deproteinised bran (DSDPB). A response surface methodology was used to obtain the optimal extraction conditions. The experimental data fit well with the predicted values and the model adequately represented the actual relationship among the measured parameters. The extraction yield and AX content in the extract under optimal conditions (double-enzyme dose of 920 U/g, pH of 3.0, extraction temperature of 35.0 °C; extraction time of 6 h; and DSDPB to liquid ratio of 1:30) were 40.73 ± 0.09% and 75.88 ± 0.11%, respectively. The double-enzymatic extraction method of AX from fresh corn fibre was more efficient than the chemical method.



中文翻译:

鲜玉米纤维双酶法提取阿拉伯木聚糖工艺优化

阿拉伯木聚糖 (AX) 的酶法提取是一种有吸引力且环保的提取选择,其中技术考虑(产量和纯度)与环境问题相结合。Amano HC 90 和纤维素酶结合使用,以评估它们对从脱淀粉、脱蛋白麸皮 (DSDPB) 中提取 AX 的交互作用。响应面方法用于获得最佳提取条件。实验数据与预测值吻合良好,模型充分反映了实测参数之间的实际关系。最佳条件下(双酶剂量920 U/g,pH 3.0,提取温度35.0°C;提取时间6 h;DSDPB液比1:30)提取率和提取液中AX含量分别为 40.73 ± 0.09% 和 75.88 ± 0.11%。

更新日期:2020-05-13
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