The rate of drug resistance strains increased drastically over the past several decades, which forced researchers to develop new drugs to treat these pathogens. In the present study by the use of well diffusion method, the antimicrobial activity of Citrus sinensis and its synergism with chitosan modified compound (CMC) were checked. The multidrug resistant bacterial strains Pseudomonas aeruginosa, Escherichia coli, Staphylococcusaureus, vancomycin-resistant Staphylococcus aureus (VRSA), Methicillin-resistant S. aureus (MRSA), Acinetobacter baumannii, VRSA and coagulase negative, and methicillin-resistant and vancomycin sensitive S. aureus were used against the different solvents of C. sinensis (seeds, leaves, stem, juice, fruit peel and C. sinensis peels) i.e. methanol and ethyl acetate to check its anti-MDR activity. Furthermore, methanolic and ethyl acetate extracts were then tagged with CMC and the results were compared. Out of two solvents, the highest activity was shown by ethyl acetate extracts followed by methanolic extracts and the formulation of the C. sinensis and CMC showed even more activity than simple extracts. Ethyl acetate extract of leaves of C. sinensis, tagged with CMC illustrates highest activity zone i.e. 53.3 mm against MDR E. coli while simple ethyl acetate C. sinensis leaf extract against A. baumannii showed the highest inhibition zone of 58.5 mm at the concentration of 100 mg/ml. Consequently, the C. sinensis could be a possible solution against superbugs.

在过去的几十年中，耐药菌株的发病率急剧上升，这迫使研究人员开发出新的药物来治疗这些病原体。在本研究中，采用孔扩散法研究了柑橘的抗菌活性及其与壳聚糖修饰化合物（CMC）的协同作用。多重耐药菌菌株铜绿假单胞菌，大肠杆菌，金黄色葡萄球菌，耐万古霉素的金黄色葡萄球菌（VRSA），耐甲氧西林的金黄色葡萄球菌（MRSA），鲍曼不动杆菌，VRSA和凝固酶阴性，耐甲氧西林使用金黄色葡萄球菌对中华C的不同溶剂（种子，叶片，茎，汁，果皮和中华C的果皮）即甲醇和乙酸乙酯进行检测，以检查其抗MDR活性。此外，然后用CMC对甲醇和乙酸乙酯提取物进行标记，并对结果进行比较。在两种溶剂中，活性最高的是乙酸乙酯提取物，其次是甲醇提取物，而中华。和CMC的配方比简单的提取物具有更高的活性。用CMC标记的中华C叶片的乙酸乙酯提取物表现出最高的抗MDR大肠杆菌活性区，即53.3 mm，而简单的乙酸乙酯中华。。在100 mg / ml的浓度下，针对鲍曼不动杆菌的叶提取物显示出58.5 mm的最高抑制区。因此，中华绒螯蟹可能是对抗超级细菌的可能解决方案。